Characterization of a novel plant acyl-CoA synthetase that is expressed in lipogenic tissues of Brassica napus L.

被引：34

作者：

Pongdontri, P

Hills, M

机构：

[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

[2] Khon Kaen Univ, Fac Sci, Dept Biochem, Khon Kaen 40002, Thailand

来源：

PLANT MOLECULAR BIOLOGY | 2001年 / 47卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

developing seed; embryo; fatty acid; lipid synthesis; long-chain acyl; CoA synthetase; membrane; oilseed rape;

D O I：

10.1023/A:1013652014744

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding a novel isoform of acyl-CoA synthetase (ACS6) was isolated from embryos of oilseed rape. Homology searches show it is most closely related to ACS4 from rat and human brain rather than the other oilseed rape ACSs. The ACS6 is strongly expressed in embryos and flowers, tissues of Brassica napus that synthesize lipids at high rates. The activity of recombinantly expressed ACS6 was recovered in the insoluble fraction (214 000 x g, 1 h pellet). CHAPS-solubilized recombinant ACS6 protein preferred utilising long-chain fatty acids that contained a cis-9 double bond, i.e. palmitoleic, oleic, linoleic and linolenic acids. Western blot analysis showed that the ACS6 protein is membrane-bound.

引用

页码：717 / 726

页数：10

共 37 条

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