Canine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells

被引:29
作者
Bismarck, Doris [1 ]
Schuetze, Nicole [1 ]
Moore, Peter [2 ]
Buettner, Mathias [3 ]
Alber, Gottfried [1 ]
v. Buttlar, Heiner [1 ]
机构
[1] Univ Leipzig, Coll Vet Med, Inst Immunol, D-04103 Leipzig, Germany
[2] Univ Calif Davis, Sch Vet Med, Dept Vet Pathol Microbiol & Immunol, Davis, CA 95616 USA
[3] Bavarian Hlth & Food Safety Author, D-85764 Oberschleissheim, Germany
关键词
Dog; Peripheral blood; CD4(+)CD8(+) T cells; Viral re-stimulation; Activated phenotype; CD8; composition; FLOW-CYTOMETRIC ANALYSIS; FUNCTIONAL-CHARACTERIZATION; MONOCLONAL-ANTIBODIES; PHENOTYPIC ANALYSIS; MEASLES-VIRUS; LYMPHOCYTES; ANTIGENS; EXPRESSION; LEUKOCYTES; EPITOPES;
D O I
10.1016/j.vetimm.2012.06.014
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In dogs a CD4(+)CD8(+) double positive T cell subpopulation exists that has not been phenotypically defined yet. We demonstrate that canine CD4(+)CD8(+) T cells are mature CD1a(-) and TCR alpha beta(+) T cells. To analyse the activation potential of CD4(+)CD8(+). T cells, PBMC from dogs vaccinated against canine distemper virus (CDV) were re-stimulated with CDV. Upon antigen-specific stimulation, the CD4(+)CD8(+) T cell fraction increases and consists nearly exclusively of proliferated cells. Similarly, other features of activated effector/memory T cells such as up-regulation of CD25 and MHC-II as well as down-regulation of CD62L (L-selectin) were observed in CD4(+)CD8(+) T cells after stimulation. Canine CD4(+)CD8(+). T cells are less abundant, but more heterogeneous than porcine ones, comprising a small proportion expressing the beta chain of CD8 in addition to the CD8 alpha chain, like human CD4(+)CD8(+) T cells. In summary, this analysis provides the basis for functional characterisation of the in vivo relevance of CD4(+)CD8(+) T cells in T-cell mediated immunity. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:157 / 166
页数:10
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