Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

被引：19

作者：

Dvorak, B

Stephana, AL

Holubec, H

Williams, CS

Philipps, AF

Koldovskoy, O

机构：

[1] UNIV ARIZONA,DEPT PEDIAT,TUCSON,AZ 85724

[2] UNIV ARIZONA,DEPT NUTR SCI,TUCSON,AZ 85724

[3] UNIV ARIZONA,DEPT MED,TUCSON,AZ 85724

来源：

FEBS LETTERS | 1996年 / 388卷 / 2-3期

关键词：

insulin-like growth factor; gastrointestinal tract; messenger RNA; in situ hybridization; competitive polymerase chain reaction;

D O I：

10.1016/0014-5793(96)00495-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied, A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups, Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings, rising an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats, By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.

引用

页码：155 / 160

页数：6

共 39 条

[1] INSULIN-LIKE GROWTH FACTOR-I MESSENGER RIBONUCLEIC-ACIDS WITH ALTERNATIVE 5'-UNTRANSLATED REGIONS ARE DIFFERENTIALLY EXPRESSED DURING DEVELOPMENT OF THE RAT [J].