Chloroplast F0F1 ATP synthase imaged by atomic force microscopy

被引:26
作者
Neff, D
Tripathi, S
Middendorf, K
Stahlberg, H
Butt, HJ
Bamberg, E
Dencher, NA
机构
[1] TECH UNIV DARMSTADT, INST BIOCHEM, PHYS BIOCHEM ABT, D-64287 DARMSTADT, GERMANY
[2] MAX PLANCK INST BIOPHYS, D-60596 FRANKFURT, GERMANY
[3] UNIV LAUSANNE, LAB ANAL ULTRASTRUCT, CH-1015 LAUSANNE, SWITZERLAND
基金
瑞士国家科学基金会;
关键词
D O I
10.1006/jsbi.1997.3891
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The F0F1 ATP synthase of chloroplasts was imaged using atomic force microscopy (AFM) in contact mode under physiological conditions. Chloroplast (CF0F1) ATP synthases were reconstituted into liposomes, Liposomes were adsorbed on a mica surface where they spread and formed lipid bilayers containing CF0F1 ATP synthases which could be imaged. From these reconstituted CF0F1 ATP synthases, the CF1 part could be removed either by application of a chemical denaturant or less efficiently by mechanical stripping with the AFM tip. Embedded in the lipid bilayer were seen ring-like structures with a central dimple with outer diameters of 20 +/- 3 nm (chemical denaturant) and ca, 7 nm (mechanical stripping), respectively, Ring-like structures were also observed in a protein-free lipid bilayer, These had diameters of 30 +/- 5 nm and could be clearly distinguished from the structures observed after mechanical stripping, Hence, the ring-like structures observed after mechanical stripping might represent the intrinsic membrane domain CF0 or the oligomer of its subunit III, In addition, isolated CF1 adsorbed directly onto the mica surface was imaged, In accordance with the size known from electron microscopy, a diameter of 13 +/- 4 nm was measured, (C) 1997 Academic Press.
引用
收藏
页码:139 / 148
页数:10
相关论文
共 46 条
[1]   STRUCTURE AT 2.8-ANGSTROM RESOLUTION OF F1-ATPASE FROM BOVINE HEART-MITOCHONDRIA [J].
ABRAHAMS, JP ;
LESLIE, AGW ;
LUTTER, R ;
WALKER, JE .
NATURE, 1994, 370 (6491) :621-628
[2]   CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].
ADRIAN, M ;
DUBOCHET, J ;
LEPAULT, J ;
MCDOWALL, AW .
NATURE, 1984, 308 (5954) :32-36
[3]  
[Anonymous], SURF SCI REP
[4]  
Apell Hans-Juergen, 1993, P275
[5]  
BINDER A, 1978, J BIOL CHEM, V253, P3094
[6]   ATOMIC FORCE MICROSCOPE [J].
BINNIG, G ;
QUATE, CF ;
GERBER, C .
PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933
[7]  
BIRKENHAGER R, 1995, EUR J BIOCHEM, V230, P58, DOI 10.1111/j.1432-1033.1995.0058i.x
[8]  
BOEKEMA EJ, 1988, Z NATURFORSCH C, V43, P219
[9]   ELECTRON CRYOMICROSCOPY OF 2-DIMENSIONAL CRYSTALS OF THE H+-ATPASE FROM CHLOROPLASTS [J].
BOTTCHER, B ;
GRABER, P ;
BOEKEMA, EJ ;
LUCKEN, U .
FEBS LETTERS, 1995, 373 (03) :262-264
[10]   The structure of the H+-ATPase from chloroplasts by electron cryomicroscopy [J].
Bottcher, B ;
Lucken, U ;
Graber, P .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1995, 23 (04) :780-785