Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs

被引：126

作者：

Jonas, D

Speck, M

Daschner, FD

Grundmann, H

机构：

[1] Univ Hosp Freiburg, Inst Environm Med & Hosp Epidemiol, Natl Reference Ctr Hyg, D-79106 Freiburg, Germany

[2] Univ Nottingham Hosp, Queens Med Ctr, Dept Microbiol, Nottingham NG7 2UH, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 05期

关键词：

D O I：

10.1128/JCM.40.5.1821-1823.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.

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页码：1821 / 1823

页数：3

相关论文

共 19 条

[1] Detection of the mec-A gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low-level methicillin resistance [J].

Bignardi, GE ;

Woodford, N ;

Chapman, A ;

Johnson, AP ;

Speller, DCE .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1996, 37 (01) :53-63

[2] Evaluation of MRSA-screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus [J].

Cavassini, M ;

Wenger, A ;

Jaton, K ;

Blanc, DS ;

Bille, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (05) :1591-1594

[3] A MAJOR OUTBREAK OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS CAUSED BY A NEW PHAGE-TYPE (EMRSA-16) [J].

COX, RA ;

CONQUEST, C ;

MALLAGHAN, C ;

MARPLES, RR .

JOURNAL OF HOSPITAL INFECTION, 1995, 29 (02) :87-106

[4]

Deplano Ariane, 1998, V15, P431

[5] Comparison of ATB Staph, Rapid ATB Staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA [J].

Frebourg, NB ;

Nouet, D ;

Lemée, L ;

Martin, E ;

Lemeland, JF .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) :52-57

[6] MULTIPLEX PCR FOR IDENTIFICATION OF METHICILLIN-RESISTANT STAPHYLOCOCCI IN THE CLINICAL LABORATORY [J].

GEHA, DJ ;

UHL, JR ;

GUSTAFERRO, CA ;

PERSING, DH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (07) :1768-1772

[7] COMPETITIVE EXCLUSION PRINCIPLE [J].

HARDIN, G .

SCIENCE, 1960, 131 (3409) :1292-1297

[8] Evaluation of the mecA femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus [J].

Jonas, D ;

Grundmann, H ;

Hartung, D ;

Daschner, FD ;

Towner, KJ .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1999, 18 (09) :643-647

[9] DETECTION OF MECA, FEMA, AND FEMB GENES IN CLINICAL STRAINS OF STAPHYLOCOCCI USING POLYMERASE CHAIN-REACTION [J].

KOBAYASHI, N ;

WU, H ;

KOJIMA, K ;

TANIGUCHI, K ;

URASAWA, S ;

UEHARA, N ;

OMIZU, Y ;

KISHI, Y ;

YAGIHASHI, A ;

KUROKAWA, I .

EPIDEMIOLOGY AND INFECTION, 1994, 113 (02) :259-266

[10]

Louie L, 2000, J CLIN MICROBIOL, V38, P2170