Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR

被引：33

作者：

De Medici, D ^{[1
]}

Croci, L ^{[1
]}

Di Pasquale, S ^{[1
]}

Fiore, A ^{[1
]}

Toti, L ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Alimenti, I-00161 Rome, Italy

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2001年 / 33卷 / 05期

关键词：

D O I：

10.1046/j.1472-765X.2001.01018.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: The objective of this study was to determine the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. Methods and Results: One hundred and forty-two mollusc samples were analysed for the presence of viral HAV-RNA using RT-nested-PCR; positive samples were then analysed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were positive for the presence of infectious virus. Conclusions: The results demonstrate the validity of the screening method (RT-nested-PCR) and the necessity of applying a method that is capable of detecting the presence of infectious HAV. Significance and Impact of the Study: The study demonstrates that in any case, to determine the safety for human consumption, the results of RT-nested-PCR must be confirmed with an integrated cell-culture PCR method.

引用

页码：362 / 366

页数：5

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