Differential gene expression of organic anion transporters in male and female rats

被引：67

作者：

Kobayashi, Y

Hirokawa, N

Ohshiro, N

Sekine, T

Sasaki, T

Tokuyama, S

Endou, H

Yamamoto, T

机构：

[1] Showa Univ, Sch Pharmaceut Sci, Dept Clin Pharm, Shinagawa Ku, Tokyo 1428555, Japan

[2] Kyorin Univ, Sch Med, Dept Pharmacol & Toxicol, Mitaka, Tokyo 1818611, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 290卷 / 01期

关键词：

organic anion transporter; sex differences; organic anion; pharmacokinetics;

D O I：

10.1006/bbrc.2001.6180

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; however, the OAT2 gene was strongly expressed in both organs of females. The abundance of rOAT2 mRNA markedly increased in castrated male rat kidney; however, treatment of castrated male rats with testosterone led to a decrease of rOAT2 mRNA. Expression of rOAT3 mRNA in intact female rats was found in the kidney and brain, whereas in males rOAT3 mRNA was also found in the liver. rOAT3 mRNA markedly decreased in the liver of castrated male rats but increased in testosterone-treated castrated male rats. Moreover, rOAT3 mRNA increased in the hypophysectomized female rat liver, indicating that rOAT3 is an inducible isoform. The present findings suggest that sex steroids play an important role in the expression and maintenance of OAT2/3 isoforms in the rat liver and kidney. Our results provide information on the differential gene expression of OAT isoforms with sex hormone dependency. (C) 2002 Elsevier Science.

引用

页码：482 / 487

页数：6

共 45 条

[1] Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1
Abe, T
Kakyo, M
Tokui, T
Nakagomi, R
Nishio, T
Nakai, D
Nomura, H
Unno, M
Suzuki, M
Naitoh, T
Matsuno, S
Yawo, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (24) : 17159 - 17163
[2] Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2
Abe, T
Kakyo, M
Sakagami, H
Tokui, T
Nishio, T
Tanemoto, M
Nomura, H
Hebert, SC
Matsuno, S
Kondo, H
Yawo, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (35) : 22395 - 22401
[3] Apiwattanakul N, 1999, MOL PHARMACOL, V55, P847
[4] Bossuyt X, 1996, J PHARMACOL EXP THER, V276, P891
[5] HEPATIC TRANSPORT-SYSTEMS REGULATING PHI, CELL-VOLUME, AND BILE SECRETION
BOYER, JL
GRAF, J
MEIER, PJ
[J]. ANNUAL REVIEW OF PHYSIOLOGY, 1992, 54 : 415 - 438
[6] Molecular cloning and characterization of multispecific organic anion transporter 4 expressed in the placenta
Cha, SH
Sekine, T
Kusuhara, H
Yu, E
Kim, JY
Kim, DK
Sugiyama, Y
Kanai, Y
Endou, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (06) : 4507 - 4512
[7] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
CHOMCZYNSKI, P
SACCHI, N
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
[8] Cloning of the full-length coding sequence of rat liver-specific organic anion transporter-1 (rlst-1) and a splice variant and partial characterization of the rat lst-1 gene
Choudhuri, S
Ogura, K
Klaassen, CD
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 274 (01) : 79 - 86
[9] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
[10] GONZALEZ FJ, 1988, PHARMACOL REV, V40, P243

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