Differential gene expression of organic anion transporters in male and female rats

被引：67

作者：

Kobayashi, Y

Hirokawa, N

Ohshiro, N

Sekine, T

Sasaki, T

Tokuyama, S

Endou, H

Yamamoto, T

机构：

[1] Showa Univ, Sch Pharmaceut Sci, Dept Clin Pharm, Shinagawa Ku, Tokyo 1428555, Japan

[2] Kyorin Univ, Sch Med, Dept Pharmacol & Toxicol, Mitaka, Tokyo 1818611, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 290卷 / 01期

关键词：

organic anion transporter; sex differences; organic anion; pharmacokinetics;

D O I：

10.1006/bbrc.2001.6180

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; however, the OAT2 gene was strongly expressed in both organs of females. The abundance of rOAT2 mRNA markedly increased in castrated male rat kidney; however, treatment of castrated male rats with testosterone led to a decrease of rOAT2 mRNA. Expression of rOAT3 mRNA in intact female rats was found in the kidney and brain, whereas in males rOAT3 mRNA was also found in the liver. rOAT3 mRNA markedly decreased in the liver of castrated male rats but increased in testosterone-treated castrated male rats. Moreover, rOAT3 mRNA increased in the hypophysectomized female rat liver, indicating that rOAT3 is an inducible isoform. The present findings suggest that sex steroids play an important role in the expression and maintenance of OAT2/3 isoforms in the rat liver and kidney. Our results provide information on the differential gene expression of OAT isoforms with sex hormone dependency. (C) 2002 Elsevier Science.

引用

页码：482 / 487

页数：6

共 45 条

[31] Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3)
Race, JE
Grassl, SM
Williams, WJ
Holtzman, EJ
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 255 (02) : 508 - 514
[32] PIROXICAM AND NAPROXEN PLASMA-CONCENTRATIONS IN PATIENTS WITH OSTEOARTHRITIS - RELATION TO AGE, SEX, EFFICACY AND ADVERSE EVENTS
RUGSTAD, HE
HUNDAL, O
HOLME, I
HERLAND, OB
HUSBY, G
GIERCKSKY, KE
[J]. CLINICAL RHEUMATOLOGY, 1986, 5 (03) : 389 - 398
[33] Cloning and functional characterization of a novel rat organic anion transporter mediating basolateral uptake of methotrexate in the kidney
Saito, H
Masuda, S
Inui, K
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) : 20719 - 20725
[34] Identification of multispecific organic anion transporter 2 expressed predominantly in the liver
Sekine, T
Cha, SH
Tsuda, M
Apiwattanakul, N
Nakajima, N
Kanai, Y
Endou, H
[J]. FEBS LETTERS, 1998, 429 (02) : 179 - 182
[35] Expression cloning and characterization of a novel multispecific organic anion transporter
Sekine, T
Watanabe, N
Hosoyamada, M
Kanai, Y
Endou, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (30) : 18526 - 18529
[36] SIMONSON GD, 1994, J CELL SCI, V107, P1065
[37] BIOCHEMICAL BASIS OF SEX-DIFFERENCES IN DRUG-METABOLISM
SKETT, P
[J]. PHARMACOLOGY & THERAPEUTICS, 1988, 38 (03) : 269 - 304
[38] Isolation of a family of organic anion transporters from human liver and kidney
Sun, W
Wu, RR
van Poelje, PD
Erion, MD
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 283 (02) : 417 - 422
[39] Expression closing and characterization of ROAT1 - The basolateral organic anion transporter in rat kidney
Sweet, DH
Wolff, NA
Pritchard, JB
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (48) : 30088 - 30095
[40] Molecular and functional identification of sodium ion-dependent, high affinity human carnitine transporter OCTN2
Tamai, I
Ohashi, R
Nezu, J
Yabuuchi, H
Oku, A
Shimane, M
Sai, Y
Tsuji, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (32) : 20378 - 20382

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