Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor

被引：87

作者：

Mello, LD

Hernandez, S

Marrazza, G

Mascini, M

Kubota, LT

机构：

[1] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP, Brazil

[2] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Santa Fe, Argentina

[3] Univ Florence, Dipartimento Chim, I-50019 Sesto Fioentino, Firenze, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 2006年 / 21卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

DNA biosensor; screen-printed electrodes; antioxidant properties;

D O I：

10.1016/j.bios.2005.05.012

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

In this work, the results of a method based on an electrochemical biosensor to detect DNA damage in vitro for the evaluation of the antioxidant properties of plant extracts are reported. The biosensor consisted of a dsDNA immobilized on a screen-printed electrode surface (SPE). DNA damage was promoted by the generation of the (OH)-O-center dot radicals via Fenton-type reaction. The interaction of the radical species with immobilised DNA in the absence and presence of antioxidants was evaluated by means of changes in the guanine oxidation peak obtained by square wave voltammetry. The results demonstrated that the DNA-based biosensor is suitable as a rapid screening test for the evaluation of antioxidant properties of samples. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1374 / 1382

页数：9

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