Effects of contamination of blood specimens with liquid potassium-EDTA anticoagulant

被引：29

作者：

Davidson, DF ^{[1
]}

机构：

[1] Crosshouse Hosp, Biochem Dept, Kilmarnock KA2 0BE, Scotland

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 2002年 / 39卷

关键词：

D O I：

10.1258/0004563021901973

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background Use of the anticoagulant EDTA, in high-concentration liquid form, in blood collection tubes can lead to cross-contamination of routine biochemistry specimens. Methods In the present study, tests affected by EDTA in the sample were potassium, calcium, magnesium, unsaturated iron-binding capacity, bicarbonate, aspartate transaminase, alanine transaminase, lactate dehydrogenase, creatine kinase, alkaline phosphatase and amylase. The likely mechanisms are discussed. Conclusions In the interests of best practice, the identification of subtle contamination of blood specimens for biochemical analysis requires a sensitive method for measurement of EDTA itself.

引用

页码：273 / 280

页数：8

共 37 条

[1]

CERIOTTI F, 1980, CLIN CHEM, V26, P327

[2] ACTION OF EDTA ON HUMAN ALKALINE PHOSPHATASES [J].

CONYERS, RAJ ;

BIRKETT, DJ ;

NEALE, FC ;

POSEN, S ;

BRUDENEL.J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 139 (02) :363-&

[3] SOME OBSERVATIONS ON THE NATURE OF THE CALCIUM-CRESOLPHTHALEIN COMPLEXONE REACTION AND ITS RELEVANCE TO THE CLINICAL LABORATORY [J].

CORNS, CM ;

LUDMAN, CJ .

ANNALS OF CLINICAL BIOCHEMISTRY, 1987, 24 :345-351

[4]

CUCCHERINI B, 1983, J LAB CLIN MED, V102, P370

[5] POSSIBLE EFFECT OF BILIRUBIN CONCENTRATION ON THE INVITRO LABILITY OF CREATINE-KINASE DURING STORAGE [J].

DAVIDSON, DF ;

HAINSWORTH, IR ;

ROWAN, R ;

KOUSOUROU, G ;

COLGAN, M .

ANNALS OF CLINICAL BIOCHEMISTRY, 1981, 18 (MAY) :185-186

[6] MEASUREMENT OF SERUM ALKALINE-PHOSPHATASE [J].

DAVIDSON, DF .

ENZYME AND MICROBIAL TECHNOLOGY, 1979, 1 (01) :9-14

[7] THE DEVELOPMENT OF COMPUTER-PROGRAMS TO PREDICT AND MINIMIZE THE EFFECT OF SAMPLE PYRUVATE ON ASPARTATE-AMINOTRANSFERASE ASSAYS [J].

DAVIDSON, DF .

ANNALS OF CLINICAL BIOCHEMISTRY, 1990, 27 :244-252

[8] A SYSTEMATIC EVALUATION OF BATHOPHENANTHROLINE, FERROZINE AND FERENE IN AN ICSH-BASED METHOD FOR THE MEASUREMENT OF SERUM IRON [J].

DERMAN, DP ;

GREEN, A ;

BOTHWELL, TH ;

GRAHAM, B ;

MCNAMARA, L ;

MACPHAIL, AP ;

BAYNES, RD .

ANNALS OF CLINICAL BIOCHEMISTRY, 1989, 26 :144-147

[9] RECOMMENDATIONS OF THE INTERNATIONAL COUNCIL FOR STANDARDIZATION IN HEMATOLOGY FOR ETHYLENEDIAMINETETRAACETIC ACID ANTICOAGULATION OF BLOOD FOR BLOOD-CELL COUNTING AND SIZING [J].

ENGLAND, JM ;

ROWAN, RM ;

VANASSENDELFT, OW ;

BULL, BS ;

COULTER, W ;

FUJIMOTO, K ;

GRONER, W ;

RICHARDSONJONES, A ;

KLEE, G ;

KOEPKE, JA ;

LEWIS, SM ;

MCLAREN, CE ;

SHINTON, NK ;

TATSUMI, N ;

VERWILGHEN, RL .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1993, 100 (04) :371-372

[10] SPURIOUS INCREASE IN PLASMA POTASSIUM CONCENTRATION AND REDUCTION IN PLASMA CALCIUM DUE TO INVITRO CONTAMINATION WITH LIQUID POTASSIUM EDETIC ACID AT PHLEBOTOMY [J].

FITZPATRICK, MF ;

NEWELL, J ;

GRIMES, H ;

EGAN, E .

JOURNAL OF CLINICAL PATHOLOGY, 1987, 40 (05) :588-588

← 1 2 3 4 →