The glycosylation site in the envelope protein of West Nile virus (Sarafend) plays an important role in replication and maturation processes

被引：36

作者：

Li, J ^{[1
]}

Bhuvanakantham, R ^{[1
]}

Howe, J ^{[1
]}

Ng, ML ^{[1
]}

机构：

[1] Natl Univ Singapore, Flavivirol Lab, Dept Microbiol, Singapore 117597, Singapore

来源：

JOURNAL OF GENERAL VIROLOGY | 2006年 / 87卷

关键词：

D O I：

10.1099/vir.0.81320-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The complete genome of West Nile (Sarafend) virus [WN(S)V] was sequenced. Phylogenetic trees utilizing the complete genomic sequence, capsid gene, envelope gene and NS5 gene/3' untranslated region of WN(S)V classified WN(S)V as a lineage 11 virus. A full-length infectious clone of WN(S)V with a point mutation in the glycosylation site of the envelope protein (pWNS-S154A) was constructed. Both growth kinetics and the mode of maturation were affected by this mutation. The titre of the pWNS-S154A virus was lower than the wild-type virus. This defect was corrected by the expression of wild-type envelope protein in trans. The pWNS-S154A virus matured intracellularly instead of at the plasma membrane as shown for the parental WN(S)V.

引用

页码：613 / 622

页数：10

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