Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers

被引：65

作者：

Ravelet, Corinne

Grosset, Catherine

Peyrin, Eric ^{[1
]}

机构：

[1] Inst Chim Mol Grenoble, CNRS, UMR 5063, Dept Pharmacochim Mol, FR-2607 Grenoble, France

[2] Univ Grenoble 1, UFR Pharm Grenoble, F-38240 Meylan, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1117卷 / 01期

关键词：

liquid chromatography; electrochromatography; capillary electrophoresis; DNA and RNA aptamers;

D O I：

10.1016/j.chroma.2006.03.101

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of the systematic evolution of ligands by exponential enrichment (SELEX) process has allowed the isolation of oligonucleotide sequences (referred as aptamers) with the capacity to recognize various classes of target molecules with high affinity and specificity. At the present time, various analytical aptamer-based formats have been developed as suitable tools for the detection and quantification of numerous targets, possessing many potential advantages over the most popular antibody technology. In this review, the aptamer applicability to the separation, purification and quantification of various analytes by liquid chromatography, capillary electrophoresis and electrochromatography is addressed. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1 / 10

页数：10

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