Preparative separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from crude alkaloids of sample Aconitum sinomontanum Nakai by high-speed counter-current chromatography

被引：89

作者：

Yang, FQ ^{[1
]}

Ito, Y ^{[1
]}

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 943卷 / 02期

关键词：

Aconitum sinomontanum; counter-current chromatography; preparative chromatography; alkaloids; lappaconitine; ranaconitine; deacetyllappaconitine; deacetylranaconitine;

D O I：

10.1016/S0021-9673(01)01464-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Analytical high-speed counter-current chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate alkaloids from Aconitum sinomontanum Nakai. The optimum solvent systems CHCl3-MeOH-0.3 M/0.2 M HCl (4:1.5:2, v/v) thus obtained led to the successful separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from 60 to 500 mg of crude alkaloid sample by preparative HSCCC separation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：219 / 225

页数：7

共 13 条

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The effects of Aconitum alkaloids on the central nervous system [J].