Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus

被引：190

作者：

Mouaikel, J ^{[1
]}

Verheggen, C ^{[1
]}

Bertrand, E ^{[1
]}

Tazi, J ^{[1
]}

Bordonné, R ^{[1
]}

机构：

[1] Inst Mol Genet, IFR 24, CNRS, UMR 5535, F-34000 Montpellier, France

来源：

MOLECULAR CELL | 2002年 / 9卷 / 04期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1016/S1097-2765(02)00484-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The m(7)G caps of most spliceosomal snRNAs and certain snoRNAs are converted posttranscriptionally to 2,2,7-trimethylguanosine (m(3)G) cap structures. Here, we show that yeast Tgs1p, an evolutionarily conserved protein carrying a signature of S-AdoMet methyltransferase, is essential for hypermethylation of the m(7)G caps of both snRNAs and snoRNAs. Deletion of the yeast TGS1 gene abolishes the conversion of the m(7)G to m(3)G caps and produces a cold-sensitive splicing defect that correlates with the retention of U1 snRNA in the nucleolus. Consistently, Tgs1p is also localized in the nucleolus. Our results suggest a trafficking pathway in which yeast snRNAs and snoRNAs cycle through the nucleolus to undergo m(7)G cap hypermethylation.

引用

页码：891 / 901

页数：11

共 46 条

[41] A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP [J].

Watkins, NJ ;

Ségault, V ;

Charpentier, B ;

Nottrott, S ;

Fabrizio, P ;

Bachi, A ;

Wilm, M ;

Rosbash, M ;

Branlant, C ;

Lührmann, R .

CELL, 2000, 103 (03) :457-466

[42] In vitro reconstitution of mammalian U1 snRNPs active in splicing: The U1-C protein enhances the formation of early (E) spliceosomal complexes [J].

Will, CL ;

Rumpler, S ;

Gunnewiek, JK ;

vanVenrooij, WJ ;

Luhrmann, R .

NUCLEIC ACIDS RESEARCH, 1996, 24 (23) :4614-4623

[43] Spliceosomal UsnRNP biogenesis, structure and function [J].

Will, CL ;

Lührmann, R .

CURRENT OPINION IN CELL BIOLOGY, 2001, 13 (03) :290-301

[44]

Yu Y-T., 1999, RNA WORLD, V37, P487

[45] Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing [J].

Yu, YT ;

Shu, MD ;

Steitz, JA .

EMBO JOURNAL, 1998, 17 (19) :5783-5795

[46] Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function [J].

Zhu, YJ ;

Qi, C ;

Cao, WQ ;

Yeldandi, AV ;

Rao, MS ;

Reddy, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (18) :10380-10385

← 1 2 3 4 5 →