Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

被引：125

作者：

Tajima, H

Niikura, T

Hashimoto, Y

Ito, Y

Kita, Y

Terashita, K

Yamazaki, K

Koto, A

Aiso, S

Nishimoto, I ^{[1
]}

机构：

[1] Keio Univ, Sch Med, Dept Pharmacol & Neurosci, Shinjuku Ku, Tokyo 1608582, Japan

[2] Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, Tokyo 1608582, Japan

[3] Keio Univ, Sch Med, Dept Anat, Shinjuku Ku, Tokyo 1608582, Japan

[4] Saiseikai Cent Hosp, Dept Pathol, Minato Ku, Tokyo 1080073, Japan

来源：

NEUROSCIENCE LETTERS | 2002年 / 324卷 / 03期

关键词：

Humanin; Alzheimer's disease; in vivo expression; Humanin mRNA; Humanin peptide; mitochondrial 16S ribosomal RNA with a polyA tail; neurone death; rescue factor;

D O I：

10.1016/S0304-3940(02)00199-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：227 / 231

页数：5

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