Oxygen microscopy by two-photon-excited phosphorescence

被引：193

作者：

Finikova, Olga S. ^{[1
]}

Lebedev, Artem Y. ^{[1
]}

Aprelev, Alexey ^{[2
]}

Troxler, Thomas ^{[3
,4
]}

Gao, Feng ^{[3
,4
]}

Garnacho, Carmen ^{[5
]}

Muro, Silvia ^{[5
]}

Hochstrasser, Robin M. ^{[3
,4
]}

Vinogradov, Sergei A. ^{[1
]}

机构：

[1] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

[2] Drexel Univ, Dept Phys, Philadelphia, PA 19104 USA

[3] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

[4] Univ Penn, RLBL, Philadelphia, PA 19104 USA

[5] Univ Penn, Dept Pharmacol, Philadelphia, PA 19104 USA

来源：

CHEMPHYSCHEM | 2008年 / 9卷 / 12期

关键词：

dendrimer; energy transfer; oxygen; phosphorescence; porphyrin;

D O I：

10.1002/cphc.200800296

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

High-resolution images of oxygen distributions in microheterogeneous samples are obtained by two-photon loser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two-photon absorption (2PA) cross-section. In this probe, energy is harvested by a 2PA antenna, which posses excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction-limited resolution, and life-time-based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO(2) in cells.

引用

页码：1673 / 1679

页数：7

共 40 条

[1] Spare the rod and spoil the eye [J].

Arden, GB ;

Sidman, RL ;

Arap, W ;

Schlingemann, RO .

BRITISH JOURNAL OF OPHTHALMOLOGY, 2005, 89 (06) :764-769

[2] Phosphorescent oxygen sensor with dendritic protection and two-photon absorbing antenna [J].

Briñas, RP ;

Troxler, T ;

Hochstrasser, RM ;

Vinogradov, SA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (33) :11851-11862

[3] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[4]

Denk W., 2006, Handbook of Biological Confocal Microscopy, P535

[5] Singlet oxygen generation via two-photon excited FRET [J].

Dichtel, WR ;

Serin, JM ;

Edder, C ;

Fréchet, JMJ ;

Matuszewski, M ;

Tan, LS ;

Ohulchanskyy, TY ;

Prasad, PN .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (17) :5380-5381

[6]

DUSTIN ML, 1986, J IMMUNOL, V137, P245

[7] Dynamic quenching of porphyrin triplet states by two-photon absorbing dyes: Towards two-photon-enhanced oxygen nanosensors [J].

Finikova, Olga S. ;

Chen, Ping ;

Ou, Zhongping ;

Kadish, Karl M. ;

Vinogradov, Sergei A. .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2008, 198 (01) :75-84

[8] Energy and electron transfer in enhanced two-photon-absorbing systems with triplet cores [J].

Finikova, Olga S. ;

Troxler, Thomas ;

Senes, Alessandro ;

DeGrado, William F. ;

Hochstrasser, Robin M. ;

Vinogradov, Sergei A. .

JOURNAL OF PHYSICAL CHEMISTRY A, 2007, 111 (30) :6977-6990

[9] Po2 profiles near arterioles and tissue oxygen consumption in rat mesentery [J].

Golub, Aleksander S. ;

Barker, Matthew C. ;

Pittman, Roland N. .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2007, 293 (02) :H1097-H1106

[10] Phosphorescence quenching method for measurement of intracellular Po2 in isolated skeletal muscle fibers [J].

Hogan, MC .

JOURNAL OF APPLIED PHYSIOLOGY, 1999, 86 (02) :720-724

← 1 2 3 4 →