Rapid monitoring of recombinant protein products: a comparison of current technologies

被引：83

作者：

Baker, KN ^{[1
]}

Rendall, MH

Patel, A

Boyd, P

Hoare, M

Freedman, RB

James, DC

机构：

[1] Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England

[2] GlaxoSmithKline Res & Dev Ltd, Beckenham BR3 3BS, Kent, England

[3] UCL, Adv Ctr Biochem Engn, London WC1E 7JE, England

[4] Univ Queensland, Dept Chem Engn, Brisbane, Qld 4072, Australia

来源：

TRENDS IN BIOTECHNOLOGY | 2002年 / 20卷 / 04期

关键词：

D O I：

10.1016/S0167-7799(01)01914-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.

引用

页码：149 / 156

页数：8

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