Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells

被引：31

作者：

Bankapur, Aseefhali ^{[1
]}

Krishnamurthy, R. Sagar ^{[2
]}

Zachariah, Elsa ^{[1
]}

Santhosh, Chidangil ^{[1
]}

Chougule, Basavaraj ^{[2
]}

Praveen, Bhavishna ^{[1
]}

Valiathan, Manna ^{[3
]}

Mathur, Deepak ^{[1
,4
]}

机构：

[1] Manipal Univ, Ctr Atom & Mol Phys, Manipal, Karnataka, India

[2] Stempeut Res Pvt Ltd, Manipal, Karnataka, India

[3] Kasturba Med Coll & Hosp, Dept Pathol, Manipal, Karnataka, India

[4] Tata Inst Fundamental Res, Mumbai 400005, Maharashtra, India

来源：

PLOS ONE | 2012年 / 7卷 / 04期

关键词：

RED-BLOOD-CELLS; UV RESONANCE RAMAN; OXIDATIVE STRESS; IRON-OXIDE; CARBON NANOTUBES; LIVING CELLS; DNA-DAMAGE; TOXICITY; CYTOTOXICITY; TWEEZERS;

D O I：

10.1371/journal.pone.0035075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 mu g/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 mu g/ml.

引用

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