A versatile method for stable carbon isotope analysis of carbohydrates by high-performance liquid chromatography/isotope ratio mass spectrometry

被引：68

作者：

Boschker, H. T. S. ^{[1
]}

Moerdijk-Poortvliet, T. C. W. ^{[1
]}

van Breugel, P. ^{[1
]}

Houtekamer, M. ^{[1
]}

Middelburg, J. J. ^{[1
]}

机构：

[1] Ctr Estuarine & Marine Ecol, Netherlands Inst Ecol NIOO KNAW, NL-4400 AC Yerseke, Netherlands

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2008年 / 22卷 / 23期

关键词：

D O I：

10.1002/rcm.3804

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method to analyze stable carbon isotope ((13)C/(12)C) ratios in a variety of carbohydrates using high-performance liquid chromatography/isotope ratio mass spectrometry (HPLC/IRMS). The chromatography is based on strong anion-exchange columns with low strength NaOH eluents. An eluent concentration of 1 mM resulted in low background signals and good separation of most of the typical plant neutral carbohydrates. We also show that more strongly bound carbohydrates such as acidic carbohydrates can be separated by inclusion of NO(3)(-) as an inorganic pusher ion in the eluent. Analyses of neutral carbohydrate concentrations and their stable carbon isotope ratios are shown for plant materials and marine sediment samples both at natural abundance and for 13 C-enriched samples. The main advantage of HPLC/IRMS analysis over traditional gas chromatography based methods is that no derivatization is needed resulting in simple sample treatment and improved accuracy and reproducibility. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：3902 / 3908

页数：7

共 28 条

[1] Linkages among the bioreactivity, chemical composition, and diagenetic state of marine dissolved organic matter [J].

Amon, RMW ;

Fitznar, HP ;

Benner, R .

LIMNOLOGY AND OCEANOGRAPHY, 2001, 46 (02) :287-297

[2] Bacterial carbon sources in coastal sediments: a cross-system analysis based on stable isotope data of biomarkers [J].

Bouillon, S. ;

Boschker, H. T. S. .

BIOGEOSCIENCES, 2006, 3 (02) :175-185

[3] Liquid chromatography coupled to isotope ratio mass spectrometry:: A new perspective on honey adulteration detection [J].

Cabanero, Ana I. ;

Recio, Jose L. ;

Ruperez, Mercedes .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (26) :9719-9727

[4] DETERMINATION OF NEUTRAL SUGARS IN PLANKTON, SEDIMENTS, AND WOOD BY CAPILLARY GAS-CHROMATOGRAPHY OF EQUILIBRATED ISOMERIC MIXTURES [J].

COWIE, GL ;

HEDGES, JI .

ANALYTICAL CHEMISTRY, 1984, 56 (03) :497-504

[5] Amino acids and hexosamines as indicators of organic matter degradation state in North Sea sediments [J].

Dauwe, B ;

Middelburg, JJ .

LIMNOLOGY AND OCEANOGRAPHY, 1998, 43 (05) :782-798

[6] Daily fluctuations of exopolymers in cultures of the benthic diatoms Cylindrotheca closterium and Nitzschia sp (Bacillariophyceae) [J].

de Brouwer, JFC ;

Stal, LJ .

JOURNAL OF PHYCOLOGY, 2002, 38 (03) :464-472

[7] The dynamics of neutral sugars in the rhizosphere of wheat.: An approach by 13C pulse-labelling and GC/C/IRMS [J].

Derrien, D ;

Marol, C ;

Balesdent, J .

PLANT AND SOIL, 2004, 267 (1-2) :243-253

[8] Measurement of the 13C/12C ratio of soil-plant individual sugars by gas chromatography/combustion/isotope-ratio mass spectrometry of silylated derivatives [J].

Derrien, D ;

Balesdent, J ;

Marol, C ;

Santaella, C .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (23) :2626-2631

[9] CARBOHYDRATE ANALYSIS OF WATER-SOLUBLE URONIC ACID-CONTAINING POLYSACCHARIDES WITH HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY USING METHANOLYSIS COMBINED WITH TFA HYDROLYSIS IS SUPERIOR TO 4 OTHER METHODS [J].

DERUITER, GA ;

SCHOLS, HA ;

VORAGEN, AGJ ;

ROMBOUTS, FM .

ANALYTICAL BIOCHEMISTRY, 1992, 207 (01) :176-185

[10] Compound-specific δ13C analysis of individual amino sugars -: a tool to quantify timing and amount of soil microbial residue stabilization [J].

Glaser, B ;

Gross, S .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (11) :1409-1416

← 1 2 3 →