IDENTIFICATION AND IN VIVO ANALYSIS OF MURINE HEMATOPOIETIC STEM CELLS

被引：3

作者：

Avagyan, Serine ^{[1
,2
]}

Amrani, Yacine M. ^{[1
,2
]}

Snoeck, Hans-Willem ^{[1
,2
]}

机构：

[1] Mt Sinai Sch Med, Dept Gene & Cell Med, New York, NY USA

[2] Mt Sinai Sch Med, Black Family Stem Cell Inst, New York, NY USA

来源：

GUIDE TO TECHNIQUES IN MOUSE DEVELOPMENT, PT A: MICE, EMBRYOS, AND CELLS, 2ND EDITION | 2010年 / 476卷

关键词：

MOUSE BONE-MARROW; CFU-S CELL; SELF-RENEWAL; COMPETITIVE REPOPULATION; PROGENITOR CELLS; HAEMOGENIC ENDOTHELIUM; LINEAGE COMMITMENT; T-CELLS; C-KIT; EXPRESSION;

D O I：

10.1016/S0076-6879(10)76023-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hematopoietic stem cells (HSCs) can self-renew and give rise to all the cells of the blood and the immune system. As they differentiate, HSCs progressively lose their self-renewal capacity and generate lineage-restricted multipotential progenitor cells that in turn give rise to mature cells. The development of rigorous quantitative in vivo assays for HSC activity combined with multicolor flow cytometry and high-speed sorting have resulted in the phenotypic definition of HSCs to virtual purity. Here, we describe the isolation and identification of HSCs by flow cytometry and the use of competitive repopulation to assess HSC number and function.

引用

页码：429 / 447

页数：19

共 97 条

[1] Upregulation of flt3 expression within the bone marrow Lin-Sca1+c-kit+ stem cell compartment is accompanied by loss of self-renewal capacity [J].

Adolfsson, J ;

Borge, OJ ;

Bryder, D ;

Theilgaard-Mönch, K ;

Åstrand-Grundström, I ;

Sitnicka, E ;

Sasaki, Y ;

Jacobsen, SEW .

IMMUNITY, 2001, 15 (04) :659-669

[2] Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential:: A revised road map for adult blood lineage commitment [J].

Adolfsson, J ;

Månsson, R ;

Buza-Vidas, N ;

Hultquist, A ;

Liuba, K ;

Jensen, CT ;

Bryder, D ;

Yang, LP ;

Borge, OJ ;

Thoren, LAM ;

Anderson, K ;

Sitnicka, E ;

Sasaki, Y ;

Sigvardsson, M ;

Jacobsen, SEW .

CELL, 2005, 121 (02) :295-306

[3] Long-term haematopoietic reconstitution by Trp53-/-p16Ink4a-/-p19Arf-/- multipotent progenitors [J].

Akala, Omobolaji O. ;

Park, In-Kyung ;

Qian, Dalong ;

Pihalja, Michael ;

Becker, Michael W. ;

Clarke, Michael F. .

NATURE, 2008, 453 (7192) :228-U12

[4] Telomerase is required to slow telomere shortening and extend replicative lifespan of HSCs during serial transplantation [J].

Allsopp, RC ;

Morin, GB ;

DePinho, R ;

Harley, CB ;

Weissman, IL .

BLOOD, 2003, 102 (02) :517-520

[5] Effect of TERT over-expression on the long-term transplantation capacity of hematopoietic stem cells [J].

Allsopp, RC ;

Morin, GB ;

Horner, JW ;

DePinho, R ;

Harley, CB ;

Weissman, IL .

NATURE MEDICINE, 2003, 9 (04) :369-U6

[6] Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche [J].

Arai, F ;

Hirao, A ;

Ohmura, M ;

Sato, H ;

Matsuoka, S ;

Takubo, K ;

Ito, K ;

Koh, GY ;

Suda, T .

CELL, 2004, 118 (02) :149-161

[7] Phenotypic characterization of murine primitive hematopoietic progenitor cells isolated on basis of aldehyde dehydrogenase activity [J].

Armstrong, L ;

Stojkovic, M ;

Dimmick, I ;

Ahmad, S ;

Stojkovic, P ;

Hole, N ;

Lako, M .

STEM CELLS, 2004, 22 (07) :1142-1151

[8] Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow [J].

Balazs, AB ;

Fabian, AJ ;

Esmon, CT ;

Mulligan, RC .

BLOOD, 2006, 107 (06) :2317-2321

[9] The earliest thymic progenitors for T cells possess myeloid lineage potential [J].

Bell, J. Jeremiah ;

Bhandoola, Avinash .

NATURE, 2008, 452 (7188) :764-U9

[10] Commitment and developmental potential of extrathymic and intrathymic T cell precursors:: Plenty to choose from [J].

Bhandoola, Avinash ;

von Boehmer, Harald ;

Petrie, Howard T. ;

Zuniga-Pfluecker, Juan Carlos .

IMMUNITY, 2007, 26 (06) :678-689

← 1 2 3 4 5 6 7 8 9 10 →