Urea determination using pH-enzyme electrode

被引：29

作者：

Koncki, R ^{[1
]}

Chudzik, A ^{[1
]}

Walcerz, I ^{[1
]}

机构：

[1] Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 21卷 / 01期

关键词：

biosensor; flow injection analysis; urea;

D O I：

10.1016/S0731-7085(99)00094-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A pH-membrane electrode with n-tridodecylamine (TDDA) as the hydrogen-ion-selective ionophore was used for the construction of a potentiometric biosensor for urea determination. The electrode was enzymatically modified by covalent binding of urease molecules directly to the surface of the potentiometric membrane. Incorporation of the urea biosensor into simple double-channel flow injection analysis (FIA) system allows reproducible urea determination in a millimolar range of concentration. The utility and limitations of the presented biosensor-FIA system for analysis of various real samples has been investigated. The system can be useful for some biomedical and pharmaceutical applications such as analyses of urine, posthaemodialysis fluid and extracts from pharmaceutical ointments containing urea. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：51 / 57

页数：7

共 13 条

[1]

[Anonymous], 1989, FLOW INJECTION ANAL

[2] PH-BASED ENZYME POTENTIOMETRIC SENSORS .1. THEORY [J].