Centriole distal appendages promote membrane docking, leading to cilia initiation

被引：300

作者：

Tanos, Barbara E. ^{[1
]}

Yang, Hui-Ju ^{[1
]}

Soni, Rajesh ^{[1
]}

Wang, Won-Jing ^{[1
]}

Macaluso, Frank P. ^{[2
]}

Asara, John M. ^{[3
,4
]}

Tsou, Meng-Fu Bryan ^{[1
,5
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Cell Biol Program, New York, NY 10065 USA

[2] Albert Einstein Coll Med, Analyt Imaging Facil, Bronx, NY 10461 USA

[3] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA

[4] Beth Israel Deaconess Med Ctr, Mass Spectrometry Core, Boston, MA 02115 USA

[5] Cornell Univ, Weill Cornell Grad Sch Med Sci, New York, NY 10065 USA

来源：

GENES & DEVELOPMENT | 2013年 / 27卷 / 02期

基金：

美国国家卫生研究院;

关键词：

centriole; cilia; distal appendages; transition fibers; membrane docking; CELL-CYCLE; DUPLICATION; PROTEOMICS; TRANSPORT; BARRIER; BASE;

D O I：

10.1101/gad.207043.112

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The distal appendages (DAPs) of centrioles have been proposed to anchor cilia to the plasma membrane, but their molecular composition, assembly, and exact function in ciliogenesis remain poorly understood. Using quantitative centrosome proteomics and superresolution microscopy, we identified five DAP components, including one previously described (CEP164), one partially characterized (CEP89 [ccdc123]), and three novel (CEP83 [ccdc41], SCLT1, and FBF1) DAP proteins. Analyses of DAP assembly revealed a hierarchy. CEP83 recruits both SCLT1 and CEP89 to centrioles. Subsequent recruitment of FBF1 and CEP164 is independent of CEP89 but mediated by SCLT1. All five DAP components are essential for ciliogenesis; loss of CEP83 specifically blocks centriole-to-membrane docking. Undocked centrioles fail to recruit TTBK2 or release CP110, the two earliest modifications found on centrioles prior to cilia assembly, revealing centriole-to-membrane docking as a temporal and spatial cue promoting cilia initiation.

引用

页码：163 / 168

页数：6

共 18 条

[1] 3-DIMENSIONAL STRUCTURE OF BASAL BODY FROM RHESUS-MONKEY OVIDUCT [J].

ANDERSON, RG .

JOURNAL OF CELL BIOLOGY, 1972, 54 (02) :246-&

[2] A ciliopathy complex at the transition zone protects the cilia as a privileged membrane domain [J].

Chih, Ben ;

Liu, Peter ;

Chinn, Yvonne ;

Chalouni, Cecile ;

Komuves, Laszlo G. ;

Hass, Philip E. ;

Sandoval, Wendy ;

Peterson, Andrew S. .

NATURE CELL BIOLOGY, 2012, 14 (01) :61-U97

[3] Cilia and developmental signaling [J].

Eggenschwiler, Jonathan T. ;

Anderson, Kathryn V. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :345-373

[4] The Spinocerebellar Ataxia-Associated Gene Tau Tubulin Kinase 2 Controls the Initiation of Ciliogenesis [J].

Goetz, Sarah C. ;

Liem, Karel F., Jr. ;

Anderson, Kathryn V. .

CELL, 2012, 151 (04) :847-858

[5] Cep164, a novel centriole appendage protein required for primary cilium formation [J].

Graser, Susanne ;

Stierhof, York-Dieter ;

Lavoie, Sebastien B. ;

Gassner, Oliver S. ;

Lamla, Stefan ;

Le Clech, Mikael ;

Nigg, Erich A. .

JOURNAL OF CELL BIOLOGY, 2007, 179 (02) :321-330

[6] The Polo kinase Plk4 functions in centriole duplication [J].

Habedanck, R ;

Stierhof, YD ;

Wilkinson, CJ ;

Nigg, EA .

NATURE CELL BIOLOGY, 2005, 7 (11) :1140-1146

[7] A Septin Diffusion Barrier at the Base of the Primary Cilium Maintains Ciliary Membrane Protein Distribution [J].

Hu, Qicong ;

Milenkovic, Ljiljana ;

Jin, Hua ;

Scott, Matthew P. ;

Nachury, Maxence V. ;

Spiliotis, Elias T. ;

Nelson, W. James .

SCIENCE, 2010, 329 (5990) :436-439

[8] Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia [J].

Ishikawa, H ;

Kubo, A ;

Tsukita, S ;

Tsukita, S .

NATURE CELL BIOLOGY, 2005, 7 (05) :517-U79

[9] Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods [J].

Jakobsen, Lis ;

Vanselow, Katja ;

Skogs, Marie ;

Toyoda, Yusuke ;

Lundberg, Emma ;

Poser, Ina ;

Falkenby, Lasse G. ;

Bennetzen, Martin ;

Westendorf, Jens ;

Nigg, Erich A. ;

Uhlen, Mathias ;

Hyman, Anthony A. ;

Andersen, Jens S. .

EMBO JOURNAL, 2011, 30 (08) :1520-1535

[10] A size-exclusion permeability barrier and nucleoporins characterize a ciliary pore complex that regulates transport into cilia [J].

Kee, Hooi Lynn ;

Dishinger, John F. ;

Blasius, T. Lynne ;

Liu, Chia-Jen ;

Margolis, Ben ;

Verhey, Kristen J. .

NATURE CELL BIOLOGY, 2012, 14 (04) :431-+

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