A Septin Diffusion Barrier at the Base of the Primary Cilium Maintains Ciliary Membrane Protein Distribution

被引：364

作者：

Hu, Qicong ^{[2
]}

Milenkovic, Ljiljana ^{[3
,4
]}

Jin, Hua ^{[5
]}

Scott, Matthew P. ^{[3
,4
,6
,7
,8
,9
]}

Nachury, Maxence V. ^{[5
]}

Spiliotis, Elias T. ^{[1
]}

Nelson, W. James ^{[2
,5
]}

机构：

[1] Drexel Univ, Dept Biol, Philadelphia, PA 19104 USA

[2] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Dev Biol, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Mol & Cellular Physiol, Sch Med, Stanford, CA 94305 USA

[6] Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA

[7] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[8] Stanford Univ, Sch Med, Dept Med, Stanford, CA 94305 USA

[9] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA

来源：

SCIENCE | 2010年 / 329卷 / 5990期

关键词：

CHLAMYDOMONAS-REINHARDTII; PLASMA-MEMBRANE; COMPARTMENTALIZATION; TRANSPORT; ORGANIZATION; POLARITY;

D O I：

10.1126/science.1191054

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In animal cells, the primary cilium transduces extracellular signals through signaling receptors localized in the ciliary membrane, but how these ciliary membrane proteins are retained in the cilium is unknown. We found that ciliary membrane proteins were highly mobile, but their diffusion was impeded at the base of the cilium by a diffusion barrier. Septin 2 (SEPT2), a member of the septin family of guanosine triphosphatases that form a diffusion barrier in budding yeast, localized at the base of the ciliary membrane. SEPT2 depletion resulted in loss of ciliary membrane protein localization and Sonic hedgehog signal transduction, and inhibited ciliogenesis. Thus, SEPT2 is part of a diffusion barrier at the base of the ciliary membrane and is essential for retaining receptor-signaling pathways in the primary cilium.

引用

页码：436 / 439

页数：4

共 30 条

[1] Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast [J].