Neutrophils Transport Antigen from the Dermis to the Bone Marrow, Initiating a Source of Memory CD8+ T Cells

被引:130
作者
Duffy, Darragh [1 ,2 ]
Perrin, Helene [1 ,2 ]
Abadie, Valerie [1 ,2 ]
Benhabiles, Nora [3 ]
Boissonnas, Alexandre [1 ,2 ]
Liard, Christelle [1 ,2 ]
Descours, Benjamin [1 ,2 ]
Reboulleau, Damien [1 ,2 ]
Bonduelle, Olivia [1 ,2 ]
Verrier, Bernard [4 ]
Van Rooijen, Nico [5 ]
Combadiere, Christophe [1 ,2 ,6 ]
Combadiere, Behazine [1 ,2 ,6 ]
机构
[1] INSERM, UMR S 945, F-75013 Paris 13, France
[2] Univ Paris 06, Lab Immun & Infect, F-75013 Paris 13, France
[3] CEA Saclay DRT LIST DCSI LOAD, CEA List, F-91191 Gif Sur Yvette, France
[4] Univ Lyon 1, Ctr Natl Rech Sci, Inst Biol & Chim Prot, F-69367 Lyon 07, France
[5] Vrije Univ Amsterdam Med Ctr, Dept Mol Cell Biol & Immunol, NL-1081 BT Amsterdam, Netherlands
[6] Hop La Pitie Salpetriere, AP HP, F-75013 Paris 13, France
关键词
IN-VIVO; CHEMOKINE RECEPTOR; DENDRITIC CELLS; LYMPH-NODES; LEISHMANIA-MAJOR; IMMUNE-RESPONSE; MICE LACKING; SITE; LYMPHOCYTES; INFECTION;
D O I
10.1016/j.immuni.2012.07.015
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The bone marrow (BM) has been identified as a possible organ for T cell priming, yet the fundamental mechanisms of a polyclonal immune response in the BM remain unknown. We found that after intradermal injection of modified vaccinia Ankara virus, unexpected sources of newly primed polyclonal virus-specific CD8(+), but not CD4(+), T cells were localized in the BM and the draining lymph nodes (dLNs) prior to blood circulation. We identified neutrophils as the virus-carrier cells from the dermis to the BM. In both neutrophil-depleted and Ccr1(-/-) mice, virus-specific BM CD8(+) responses were lost. Myeloid antigen-presenting cells were required for BM CD8(+) T cell priming. A systems biology analysis of dLN and BM virus-specific CD8(+) T cells revealed distinct transcriptional and multifunctional profiles for cells primed in each organ. We provide direct evidence for how antigen is transported to the BM, providing a source of virus-specific memory CD8(+) T cells.
引用
收藏
页码:917 / 929
页数:13
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