A simple one-step real-time RT-PCR for diagnosis of dengue virus infection

被引：32

作者：

Godoy dos Santos, Harryson Wings ^{[1
]}

Ramos Silva Poloni, Telma Regina ^{[1
]}

Souza, Kelly Paula ^{[1
]}

Menjon Muller, Vanessa Danielle ^{[1
]}

Tremeschin, Flavia ^{[1
]}

Nali, Livia Christensen ^{[2
]}

Fantinatti, Leandro Ricardo ^{[2
]}

Amarilla, Alberto Anastacio ^{[2
]}

Alfonso Castro, Helda Liz ^{[1
]}

Nunes, Marcio Roberto ^{[3
]}

Casseb, Samir Mansour ^{[3
]}

Vasconcelos, Pedro Fernando ^{[3
]}

Badra, Soraya Jabur ^{[2
]}

Moraes Figueiredo, Luiz Tadeu ^{[2
]}

Aquino, Victor Hugo ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Pesquisa Virol, Ribeirao Preto, SP, Brazil

[3] Minist Saude, Secretaria Vigilancia Saude, Inst Evandro Chagas, Belem, Para, Brazil

来源：

JOURNAL OF MEDICAL VIROLOGY | 2008年 / 80卷 / 08期

关键词：

dengue; diagnosis; real-time RT-PCR;

D O I：

10.1002/jmv.21203

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dengue is the most important arbovirus disease in tropical and sub-tropical countries, and can be caused by infection with any of the four-dengue virus (DENV) serotypes. Infection with DENV can lead to a broad clinical spectrum, ranging from sub-clinical infection or an influenza-like disease known as dengue fever (DF) to a severe, sometimes fatal, disease characterized by hemorrhage and plasma leakage that can lead to shock, known as dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The diagnosis of dengue is routinely accomplished by serologic assays, such as IgM and IgG ELISAs, as well as HI tests, analyzing serum samples obtained from patients with at least 7 days of symptoms onset. These tests cannot be used for diagnosis during the early symptomatic phase. In addition, antibodies against dengue are broad reactive with other flaviviruses. Therefore, a specific diagnostic method for acute DENV infection is of great interest. In that sense, the real-time RT-PCR has become an important tool that can be used for early and specific detection of dengue virus genome in human serum samples. This study describes a simple, specific, and sensitive real-time RT-PCR for early diagnosis of dengue virus infection.

引用

页码：1426 / 1433

页数：8

共 46 条

[1] Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the experiencing primary acute phase of disease in patients or secondary infections [J].