Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana

被引：84

作者：

Carrie, Chris ^{[1
]}

Murcha, Monika W. ^{[1
]}

Kuehn, Kristina ^{[1
]}

Duncan, Owen ^{[1
]}

Barthet, Michelle ^{[2
]}

Smith, Penelope M. ^{[2
]}

Eubel, Holger ^{[1
]}

Meyer, Etienne ^{[1
]}

Day, David A. ^{[2
]}

Millar, A. Harvey ^{[1
]}

Whelan, James ^{[1
]}

机构：

[1] Univ Western Australia, ARC Ctr Excellence Plant Energy Biol, Crawley, WA 6009, Australia

[2] Univ Sydney, Sch Biol Sci, ARC Ctr Excellence Plant Energy Biol, Sydney, NSW 2006, Australia

来源：

FEBS LETTERS | 2008年 / 582卷 / 20期

基金：

澳大利亚研究理事会;

关键词：

chloroplast; mitochondria; peroxisome; dual targeting; green fluorescent protein; alternative NAD(P)H dehydrogenase;

D O I：

10.1016/j.febslet.2008.07.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found that four type II NAD(P) H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3073 / 3079

页数：7

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