Transforming carbonic anhydrase into epoxide synthase by metal exchange

被引：87

作者：

Fernandez-Gacio, Ana

Codina, Antonio

Fastrez, Jacques

Riant, Olivier

Soumillion, Patrice

机构：

[1] Univ Catholique Louvain, Inst Sci Vie, B-1348 Louvain, Belgium

[2] Univ Catholique Louvain, Dept Chim, B-1348 Louvain, Belgium

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 07期

关键词：

catalysis; chirality; epoxidation; manganese; metalloenzymes;

D O I：

10.1002/cbic.200600127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enantioselective epoxidation of styrene was observed in the presence of manganese-containing carbonic anhy arase as catalyst. The probable oxygen transfer reagent is peroxymonocarbon ate, which has a structural similarity with the hydrogenocarbonate substrate of the natural reaction. Styrene was chosen as the enzyme possesses a small hydrophobic cavity close to the active site. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：1013 / 1016

页数：4

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