A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

被引：122

作者：

Yang, Jia-Shu ^{[1
,2
]}

Gad, Helge ^{[3
]}

Lee, Stella Y. ^{[1
,2
]}

Mironov, Alexander ^{[3
]}

Zhang, Leiliang ^{[1
,2
]}

Beznoussenko, Galina V. ^{[3
]}

Valente, Carmen ^{[3
]}

Turacchio, Gabriele ^{[3
]}

Bonsra, Akua N. ^{[4
,5
]}

Du, Guangwei ^{[4
,5
]}

Baldanzi, Gianluca ^{[6
]}

Graziani, Andrea ^{[6
]}

Bourgoin, Sylvain ^{[7
]}

Frohman, Michael A. ^{[4
,5
]}

Luini, Alberto ^{[3
]}

Hsu, Victor W. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Div Rheumatol Allergy & Immunol, Brigham & Womens Hosp, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA

[3] Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Chieti, Italy

[4] SUNY Stony Brook, Dept Pharmacol, Stony Brook, NY 11794 USA

[5] SUNY Stony Brook, Ctr Dev Genet, Stony Brook, NY 11794 USA

[6] Univ Piemonte Orientale, Dept Clin & Expt Med, I-28100 Novara, Italy

[7] Ctr Hosp Univ Quebec, Pavillon CHUL, Quebec City, PQ G1V 4G2, Canada

来源：

NATURE CELL BIOLOGY | 2008年 / 10卷 / 10期

基金：

加拿大健康研究院;

关键词：

D O I：

10.1038/ncb1774

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.

引用

页码：1146 / 1153

页数：8

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