Three-Dimensional Telomeric Analysis of Isolated Circulating Tumor Cells (CTCs) Defines CTC Subpopulations

被引：29

作者：

Awe, Julius Adebayo ^{[1
,2
,3
]}

Xu, Mark Chu ^{[1
]}

Wechsler, Janine ^{[4
,5
]}

Benali-Furet, Naoual ^{[4
]}

Cayre, Yvon E. ^{[4
,6
,7
]}

Saranchuk, Jeff ^{[8
]}

Drachenberg, Darrel ^{[8
]}

Mai, Sabine ^{[1
]}

机构：

[1] Univ Manitoba, Manitoba Inst Cell Biol, CancerCare Manitoba, Winnipeg, MB, Canada

[2] Univ Gothenburg, Dept Clin Genet, Inst Biomed, Sahlgrenska Acad, Gothenburg, Sweden

[3] Univ Skovde, Sch Life Sci, Syst Biol Res Ctr, Skovde, Sweden

[4] ScreenCell, Paris, France

[5] Hop Henri Mondor, Dept Pathol, F-94010 Creteil, France

[6] Hop Robert Debre, F-75019 Paris, France

[7] Univ Paris 06, Paris, France

[8] Univ Manitoba, Dept Surg, Urol Sect, Manitoba Prostate Ctr, Winnipeg, MB R3T 2N2, Canada

来源：

TRANSLATIONAL ONCOLOGY | 2013年 / 6卷 / 01期

基金：

加拿大健康研究院;

关键词：

PERIPHERAL-BLOOD; CANCER; ORGANIZATION; ARCHITECTURE; SURVIVAL; BIOMARKERS; CARCINOMA; NUCLEUS; BIOPSY; DEVICE;

D O I：

10.1593/tlo.12361

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Circulating tumor cells (CTCs) have been identified with the potential to serve as suitable biomarkers for tumor stage and progression, but the availability of effective isolation technique(s) coupled with detailed molecular characterization have been the challenges encountered in making CTCs clinically relevant. For the first time, we combined isolation of CTCs using the ScreenCell filtration technique with quantitative analysis of CTC telomeres by TeloView. This resulted in the identification and molecular characterization of different subpopulations of CTCs in the same patient. Three-dimensional (3D) telomeric analysis was carried out on isolated CTCs of 19 patients that consisted of four different tumor types, namely, prostate, colon, breast, melanoma, and one lung cancer cell line. With telomeric analysis of the filter-isolated CTCs, the level of chromosomal instability (CIN) of the CTCs can be determined. Our study shows that subpopulations of CTCs can be identified on the basis of their 3D telomeric properties.

引用

页码：51 / U184

页数：16

共 37 条

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