Electrodeposition of gold nanoparticles on indium/tin oxide electrode for fabrication of a disposable hydrogen peroxide biosensor

被引：119

作者：

Wang, Jianwen ^{[1
]}

Wang, Liping ^{[1
]}

Di, Junwei ^{[1
]}

Tu, Yifeng ^{[1
]}

机构：

[1] Suzhou Univ, Dept Chem, Suzhou 215123, Jiangsu, Peoples R China

来源：

TALANTA | 2009年 / 77卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Gold nanoparticles; Indium tin oxide; Direct electron transfer; Horseradish peroxidase; Disposable biosensor; SOL-GEL NETWORK; FILM COATED GLASS; ONE-STEP METHOD; HORSERADISH-PEROXIDASE; DIRECT ELECTROCHEMISTRY; 3RD-GENERATION BIOSENSOR; OPTICAL-PROPERTIES; CARBON ELECTRODE; GLUCOSE-OXIDASE; SENSOR;

D O I：

10.1016/j.talanta.2008.09.034

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel disposable third-generation hydrogen peroxide (H2O2)biosensor based on horseradish peroxidase (HRP) immobilized on the gold nanoparticles (AuNPs) electrodeposited indium tin oxide (ITO) electrode is investigated. The AuNPs deposited on ITO electrode were characterized by UV-vis, SEM, and electrochemical methods. The AuNPs attached on the ITO electrode surface with quasi-spherical shape and the average size of diameters was about 25 nm with a quite symmetric distribution. The direct electron chemistry of HRP was realized. and the biosensor exhibited excellent performances for the reduction of H2O2. The amperometric response to H2O2 shows a linear relation in the range from 8.0 mu mol L-1 to 3.0 mmol L-1 and a detection limit of 2 mu mol L-1 (S/N = 3). The K-M(app) value of HRP immobilized on the electrode surface was found to be 0.4 mmol L-1. The biosensor indicates excellent reproducibility, high selectivity and long-term stability. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1454 / 1459

页数：6

共 39 条

[21] Disposable biosensor based on enzyme immobilized on Au-chitosan-modified indium tin oxide electrode with flow injection amperometric analysis [J].

Lin, Jiehua ;

Qu, Wei ;

Zhang, Shusheng .

ANALYTICAL BIOCHEMISTRY, 2007, 360 (02) :288-293

[22] A facile electrochemical sensor designed from gold nanoparticles embedded in three-dimensional sol-gel network for concurrent detection of toxic chemicals [J].

Maduraiveeran, Govindhan ;

Ramaraj, Ramasamy .

ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (08) :2051-2055

[23] Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles [J].

Morrin, A ;

Wilbeer, F ;

Ngamna, O ;

Moulton, SE ;

Killard, AJ ;

Wallace, GG ;

Smyth, MR .

ELECTROCHEMISTRY COMMUNICATIONS, 2005, 7 (03) :317-322

[24]

Shipway AN, 2000, CHEMPHYSCHEM, V1, P18, DOI 10.1002/1439-7641(20000804)1:1<18::AID-CPHC18>3.0.CO

[25]

2-L

[26] On-chip amperometric measurement of quantal catecholamine release using transparent indium tin oxide electrodes [J].

Sun, XH ;

Gillis, KD .

ANALYTICAL CHEMISTRY, 2006, 78 (08) :2521-2525

[27] Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan [J].

Tangkuaram, Tanin ;

Ponchio, Chatchai ;

Kangkasomboon, Thippayawadee ;

Katikawong, Panadda ;

Veerasai, Waret .

BIOSENSORS & BIOELECTRONICS, 2007, 22 (9-10) :2071-2078

[28] Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium [J].

Wang, BQ ;

Li, B ;

Wang, ZX ;

Xu, GB ;

Wang, Q ;

Dong, SJ .

ANALYTICAL CHEMISTRY, 1999, 71 (10) :1935-1939

[29] A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized on colloidal Au modified ITO electrode [J].

Wang, L ;

Wang, EK .

ELECTROCHEMISTRY COMMUNICATIONS, 2004, 6 (02) :225-229

[30] Direct electrodeposition of gold nanoparticles onto indium/tin oxide film coated glass and its application for electrochemical biosensor [J].

Wang, Liping ;

Mao, Wei ;

Ni, Dandan ;

Di, Junwei ;

Wu, Ying ;

Tu, Yifeng .

ELECTROCHEMISTRY COMMUNICATIONS, 2008, 10 (04) :673-676

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