Combined effects of a buffer and solvent on tetramethylpyrazine formation in a 3-hydroxy-2-butanone/ammonium hydroxide system

被引：9

作者：

Huang, TC

机构：

[1] Department of Food Science and Technology, National Pingtung Polytechnic Institute, Pingtung, 912, Taiwan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 06期

基金：

美国国家科学基金会;

关键词：

tetramethylpyrazine; phosphate buffer; protic solvent; buffer capacity;

D O I：

10.1271/bbb.61.1013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phosphate buffer was found to significantly promote tetramethylpyrazine (TMP) formation in an acetoin (3-hydroxy-2-butanone)/ammonium hydroxide system. The effect of the phosphate ion on TMP formation was additive in the range of 0.05-0.2 M. The change in pH value of the system reveals that a proton-coupled redox type of reaction occurred during TMP formation, Phosphate serves both as proton donor and acceptor to facilitate proton transfer during the Schiff base formation between ammonia and 3-hydroxy-2-butanone. Protic solvents, methanol, and ethanol, were found to attract the water released from the system, The combination of a phosphate buffer and protic solvent led to the completion of TMP formation, The TMP formation mechanism in a phosphate buffer (pH 7.2) is proposed.

引用

页码：1013 / 1015

页数：3

共 21 条

[1] ASPARTAME DEGRADATION IN SOLUTION AS IMPACTED BY BUFFER TYPE AND CONCENTRATION [J].