The zebrafish genome contains two distinct selenocysteine tRNA[Ser]Sec genes

被引：15

作者：

Xu, XM

Zhou, X

Carlson, BA

Kim, LK

Huh, TL

Lee, BJ

Hatfield, DL

机构：

[1] NCI, Sect Mol Biol Selenium, Basic Res Lab, Div Basic Sci,NIH, Bethesda, MD 20892 USA

[2] Seoul Natl Univ, Inst Mol Biol & Genet, Mol Genet Lab, Seoul 151742, South Korea

[3] Kyungpook Natl Univ, Dept Genet Engn, Taegu 702702, South Korea

来源：

FEBS LETTERS | 1999年 / 454卷 / 1-2期

关键词：

zebrafish; selenocysteine; tRNA; gene; selenium;

D O I：

10.1016/S0014-5793(99)00767-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The zebrafish is widely used as a model system for studying mammalian developmental genetics and more recently, as a model system for carcinogenesis. Since there is mounting evidence that selenium can prevent cancer in mammals, including humans, we characterized the selenocysteine tRNA([Ser]Sec) gene and its product in zebrafish, Two genes for this tRNA were isolated and sequenced and were found to map at different loci within the zebrafish genome. The encoding sequences of both are identical and their flanking sequences are highly homologous for several hundred bases in both directions. The two genes likely arose from gene duplication which is a common phenomenon among many genes in this species, Tn addition, zebrafish tRNA([Ser]Sec) was isolated from the total tRNA population and shown to decode UGA in a ribosomal binding assay, (C) 1999 Federation of European Biochemical Societies.

引用

页码：16 / 20

页数：5

共 17 条

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