Use of optimised PCR methods for the detection of GLRaV3: A closterovirus associated with grapevine leafroll in Tunisian grapevine plants

被引：11

作者：

Acheche, H

Fattouch, S

M'Hirsi, S

Marzouki, N

Marrakchi, M

机构：

[1] Fac Sci Tunis, Lab Genet & Biol Mol, Tunis 1002, Tunisia

[2] Inst Natl Sci Appl & Technol, Lab Genie Biol, Tunis, Tunisia

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1999年 / 17卷 / 01期

关键词：

grapevine leafroll virus; IC-RT-PCR; mealybugs; RT-PCR;

D O I：

10.1023/A:1007541826774

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a modification and optimisation of a previously published procedure (Minafra and Hadidi, 1994) for the detection of GLRaV3 in infected grapevine plants. GLRaV3 RNA was successfully detected not only in total crude nucleic acid extracts of infected grapevine tissues but also in viruliferous mealybug extracts by IC-RT-PCR. This detection was rapid, sensitive and specific without occurrence of any background. A comparative ELISA, RT-PCR and IC-RT-PCR assays were carried out and revealed the greater sensitivity and specificity of PCR techniques.

引用

页码：31 / 42

页数：12

共 27 条

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