High prevalence of GB virus C infection in a group of Italian patients with hepatitis of unknown etiology

被引：162

作者：

Fiordalisi, G

Zanella, I

Mantero, G

Bettinardi, A

Stellini, R

Paraninfo, G

Cadeo, G

Primi, D

机构：

[1] CONSORZIO BIOTECNOL, I-25123 BRESCIA, ITALY

[2] UNIV BRESCIA, FAC MED & CHIRURG, CATTEDRA CHIM APPL SCI BIOMED, BRESCIA, ITALY

[3] SPEDALI CIVILI BRESCIA, DIV MALATTIE INFETT 1, BRESCIA, ITALY

来源：

JOURNAL OF INFECTIOUS DISEASES | 1996年 / 174卷 / 01期

关键词：

D O I：

10.1093/infdis/174.1.181

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Prevalence of the recently discovered GB virus C (GBV-C) was evaluated in a cohort of 49 Italian patients with acute or chronic hepatitis of unknown etiology (non-A-E hepatitis) and in a control group of 100 healthy blood donors. The GBV-C genomes could be detected by polymerase chain reaction (PCR) with reverse transcription in 35% of the acute and 39% of the chronic hepatitis patients; only 1 of the control subjects had a positive response. All PCR products hybridized with a specific probe in a colorimetric assay, and the analysis of the sequences of the amplified cDNAs fully confirmed the specificity of the assay. Furthermore, the alignment of the predicted translation products identified two recurrent amino acid substitutions in 6 patients, suggesting the possible existence of at least 2 different GBV-C subtypes. Thus, GBV-C may be an important agent, contributing, at least in Italy, to a significant number of the cases of hepatitis of unknown etiology.

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页码：181 / 183

页数：3

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