The CHD3 remodeler PICKLE promotes trimethylation of histone H3 lysine 27

被引：123

作者：

Zhang, Heng ^{[1
]}

Rider, Stanley Dean, Jr. ^{[1
]}

Henderson, James T. ^{[1
]}

Fountain, Matthew ^{[1
]}

Chuang, King ^{[1
]}

Kandachar, Vasundhara ^{[1
]}

Simons, Alexis ^{[1
]}

Edenberg, Howard J. ^{[2
,3
]}

Romero-Severson, Jeanne ^{[4
]}

Muir, William M. ^{[5
]}

Ogas, Joe ^{[1
]}

机构：

[1] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA

[2] Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA

[3] Indiana Univ, Sch Med, Ctr Med Genom, Indianapolis, IN 46202 USA

[4] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

[5] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2008年 / 283卷 / 33期

关键词：

D O I：

10.1074/jbc.M802129200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CHD3 proteins are ATP-dependent chromatin remodelers that contribute to repression of developmentally regulated genes in both animal and plant systems. In animals, this repression has been linked to a multiple subunit complex, Mi-2/ NuRD, whose constituents include aCHD3protein, a histone deacetylase, and a methylCpG-binding domain protein. In Arabidopsis, PICKLE ( PKL) codes for a CHD3 protein that acts during germination to repress expression of seed-associated genes. Repression of seed-associated traits is promoted in pkl seedlings by the plant growth regulator gibberellin (GA). We undertook a microarray analysis to determine how PKL and GA act to promote the transition from seed to seedling. We found that PKL and GA actin separate pathways to repress expression of seed-specific genes. Comparison of genomic datasets revealed that PKL-dependent genes are enriched for trimethylation of histone H3 lysine 27 (H3K27me3), a repressive epigenetic mark. Chromatin immunoprecipitation studies demonstrate that PKL promotes H3K27me3 in both germinating seedlings and in adult plants but do not identify a connection between PKL dependent expression and acetylation levels. Taken together, our analyses illuminate a new pathway by which CHD3 remodelers contribute to repression in eukaryotes.

引用

页码：22637 / 22648

页数：12

共 63 条

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