Supercritical fluid extraction and high-performance liquid chromatographic determination of phloroglucinols in St. John's wort (Hypericum perforatum L.)

被引：26

作者：

Cui, YY ^{[1
]}

Ang, CYW ^{[1
]}

机构：

[1] US FDA, Natl Ctr Toxicol Res, Div Chem, Jefferson, AR 72079 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 10期

关键词：

Hypericum perforatum L; St. John's wort; supercritical fluid extraction; hyperforin; adhyperforin;

D O I：

10.1021/jf011304n

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A small-scale supercritical fluid extraction (SFE) method was developed for the selective extraction of phloroglucinols from St. John's wort (SJW) leaf/flower mixtures using supercritical carbon dioxide (CO2). The extraction efficiency was investigated as influenced by pressure, temperature, time, and modifier. The optimized condition of SFE was carried out at 3.80 x 10(4) kpa (5500 psi) and 50 degreesC. Samples were held in static extraction for 10 min, followed by a dynamic extraction for 90 min at the flow rate of 1 mL/min. A simple and sensitive HPLC method was developed for the analysis of hyperforin and adhyperforin, the major phloroglucinols, in the SFE extract of SJW.

引用

页码：2755 / 2759

页数：5

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