Quantitative P-31 nuclear magnetic resonance analysis of the phospholipids of erythrocyte membranes using detergent

被引:38
作者
NouriSorkhabi, MH
Wright, LC
Sullivan, DR
Kuchel, PW
机构
[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA
[2] WESTMEAD HOSP,DEPT INFECT DIS,WESTMEAD,NSW 2145,AUSTRALIA
[3] ROYAL PRINCE ALFRED HOSP,DEPT CLIN BIOCHEM,CAMPERDOWN,NSW 2050,AUSTRALIA
关键词
D O I
10.1007/BF02522893
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
P-31 Nuclear magnetic resonance (NMR) spectra of human erythrocyte lysates dissolved in sodium cholate were acquired. The narrow resonances of phospholipids were mostly well resolved, allowing identification and accurate quantitative analysis of phospholipid classes of the erythrocyte membranes. The ether-linked phosphatidylethanolamine components of the erythrocyte membranes were identified, based on the removal of plasmalogens by acidolysis and of diacyl phospholipid species by degradation using phospholipase A(1). It was also shown that the introduction of double bonds on the acyl chains of phosphatidylcholine shifted the P-31 NMR resonances to lower frequencies. Quantitative analyses of phospholipids from the spectra were based on their apparent molar concentrations. The recoveries of phospholipids from erythrocytes were significantly higher than those using conventional extraction procedures.
引用
收藏
页码:765 / 770
页数:6
相关论文
共 28 条
[11]   CHANGES OF MEMBRANE PHOSPHOLIPID-COMPOSITION OF HUMAN ERYTHROCYTES IN HYPERLIPIDEMIAS .2. INCREASES IN DISTINCT MOLECULAR-SPECIES OF PHOSPHATIDYLETHANOLAMINE AND PHOSPHATIDYLCHOLINE CONTAINING ARACHIDONIC-ACID [J].
ENGELMANN, B ;
SCHONTHIER, UM ;
RICHTER, WO ;
DUHM, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1165 (01) :38-44
[12]   CHANGES OF MEMBRANE PHOSPHOLIPID-COMPOSITION OF HUMAN ERYTHROCYTES IN HYPERLIPIDEMIAS .1. INCREASED PHOSPHATIDYLCHOLINE AND REDUCED SPHINGOMYELIN IN PATIENTS WITH ELEVATED LEVELS OF TRIACYLGLYCEROL-RICH LIPOPROTEINS [J].
ENGELMANN, B ;
STREICH, S ;
SCHONTHIER, UM ;
RICHTER, WO ;
DUHM, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1165 (01) :32-37
[13]  
ENGEN RL, 1990, AM J VET RES, V4, P577
[14]  
FOLCH J, 1957, J BIOL CHEM, V226, P497
[15]   THE ADVANTAGES OF RAPID MULTIPULSING IN THE ROUTINE ACQUISITION OF NMR-SPECTRA [J].
HOMER, J ;
ROBERTS, JK .
JOURNAL OF MAGNETIC RESONANCE, 1989, 81 (03) :484-490
[16]  
LONDON E, 1979, J LIPID RES, V20, P408
[17]  
MUKHERJEE M, 1994, CLIN HEMORHEOL, V14, P131
[18]  
Nelson G.J., 1972, Blood Lipids and Lipo proteins: Quantitation, Composition, and Metabolism, P317
[19]   LIPID COMPOSITION OF ERYTHROCYTES IN VARIOUS MAMMALIAN SPECIES [J].
NELSON, GJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 144 (02) :221-&
[20]   Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including Australian marsupials; Quantitative P-31 NMR analysis using detergent [J].
NouriSorkhabi, MH ;
Agar, NS ;
Sullivan, DR ;
Gallagher, C ;
Kuchel, PW .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1996, 113 (02) :221-227