Novel noncoding antisense RNA transcribed from human anti-NOS2A locus is differentially regulated during neuronal differentiation of embryonic stem cells

被引:41
作者
Korneev, Sergei A. [1 ]
Korneeva, Elena I. [1 ]
Lagarkova, Marya A. [2 ]
Kiselev, Sergei L. [2 ]
Critchley, Giles [3 ]
O'Shea, Michael [1 ]
机构
[1] Univ Sussex, Sch Life Sci, Sussex Ctr Neurosci, Brighton BN1 9QG, E Sussex, England
[2] NI Vavilov Gen Genet Res Inst, Moscow 119991, Russia
[3] Hurstwood Pk Neurol Ctr, Haywards Heath RH16 4EX, W Sussex, England
基金
英国惠康基金;
关键词
antisense RNA; DNA inversion; nitric oxide; stem cells; neuronal differentiation;
D O I
10.1261/rna.1084308
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Here, we report on the discovery of a locus in the human genome, which evolved by gene duplication followed by an internal DNA inversion. This locus exhibits high sequence similarity to the gene for the inducible isoform of NOS protein (NOS2A) and is transcribed into a noncoding RNA containing a region of significant antisense homology with the NOS2A mRNA. We show that this antisense transcript (anti-NOS2A RNA) is expressed in different types of brain tumors, including meningiomas and glioblastomas. More importantly, we demonstrate that the expression profiles of the anti-NOS2A RNA and the NOS2A mRNA exhibit concurrent reciprocal changes in undifferentiated human embryonic stem cells (hESCs) and in hESCs induced to differentiate into neurogenic precursors such as neurospheres. As NOS2A has a role in neurogenesis, our results suggest that the anti-NOS2A RNA is involved in the regulation of neuronal differentiation of hESCs through the modulation of NOS2A gene expression.
引用
收藏
页码:2030 / 2037
页数:8
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