Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis

被引:323
作者
Kim, HJ
Ryu, H
Hong, SH
Woo, HR
Lim, PO
Lee, IC
Sheen, J
Nam, HG [1 ]
Hwang, I
机构
[1] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea
[2] Pohang Univ Sci & Technol, Natl Core Res Ctr Biodynam, Pohang 790784, South Korea
[3] Pohang Univ Sci & Technol, IBIO Grad Program, Pohang 790784, South Korea
[4] Cheju Natl Univ, Dept Sci Educ, Cheju 690756, South Korea
[5] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
[6] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
关键词
histidine kinase 3; cytokinin; leaf senescence;
D O I
10.1073/pnas.0505150103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cytokinins are plant hormones with profound roles in growth and development, including control of leaf longevity. Although the cytokinin signal is known to be perceived by histidine kinase receptors, the underlying molecular mechanism and specificity of the receptors leading to delayed leaf senescence have not yet been elucidated. Here, we found that AHK3, one of the three cytokinin receptors in Arabidopsis, plays a major role in controlling cytokinin-mediated leaf longevity through a specific phosphorylation of a response regulator, ARR2. This result was obtained through identification of a gain-of-function Arabidopsis mutant that shows delayed leaf senescence because of a missense mutation in the extracellular domain of AHK3. A loss-of-function mutation in AHK3, but not of the other cytokinin receptors, conferred a reduced sensitivity to cytokinin in cytokinin-dependent delay of leaf senescence and abolished cytokinin-dependent phosphorylation of ARR2. Consistently, transgenic overexpression of wild-type, but not an unphosphorylatable mutant ARR2, led to delayed senescence of leaves.
引用
收藏
页码:814 / 819
页数:6
相关论文
共 32 条
[21]   Cytokinin metabolism and action [J].
Mok, DWS ;
Mok, MC .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2001, 52 :89-118
[22]   The molecular genetic analysis of leaf senescence [J].
Nam, HG .
CURRENT OPINION IN BIOTECHNOLOGY, 1997, 8 (02) :200-207
[23]  
NELSON CJ, 1988, PLANT PHYSIOL BIOCH, V26, P543
[24]   Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis [J].
Nishimura, C ;
Ohashi, Y ;
Sato, S ;
Kato, T ;
Tabata, S ;
Ueguchi, C .
PLANT CELL, 2004, 16 (06) :1365-1377
[25]  
Nooden LD., 1988, Senescence and Aging in Plants, P1, DOI DOI 10.1016/B978-0-12-520920-5.50007-9.
[26]   A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescence [J].
Oh, SA ;
Lee, SY ;
Chung, IK ;
Lee, CH ;
Nam, HG .
PLANT MOLECULAR BIOLOGY, 1996, 30 (04) :739-754
[27]   Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana [J].
Oh, SA ;
Park, JH ;
Lee, GI ;
Paek, KH ;
Park, SK ;
Nam, HG .
PLANT JOURNAL, 1997, 12 (03) :527-535
[28]   EFFECT OF KINETIN ON PROTEIN CONTENT AND SURVIVAL OF DETACHED XANTHIUM LEAVES [J].
RICHMOND, AE ;
LANG, A .
SCIENCE, 1957, 125 (3249) :650-651
[29]   Ca2+-dependent protein kinases and stress signal transduction in plants [J].
Sheen, J .
SCIENCE, 1996, 274 (5294) :1900-1902
[30]   Two-component signal transduction [J].
Stock, AM ;
Robinson, VL ;
Goudreau, PN .
ANNUAL REVIEW OF BIOCHEMISTRY, 2000, 69 :183-215