Fluorescence activated cell sorting for enzymatic activity

被引：14

作者：

Farinas, ET ^{[1
]}

机构：

[1] New Jersey Inst Technol, Dept Chem & Environm Sci, Newark, NJ 07102 USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2006年 / 9卷 / 04期

关键词：

directed evolution; FACS; high-throughput screening; enzyme engineering;

D O I：

10.2174/138620706776843200

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Directed evolution is a reliable method for protein engineering and as a tool for investigating structure/function relationships. A key for a successful directed evolution experiment is oftentimes the screen. Fluorescence activated cell sorting (FACS) is powerful high-throughput screening approach to isolate and identify mutants from large protein libraries. FACS has been successful in isolating proteins with improved or altered binding affinity. However, FACS screening for mutants with enhanced catalytic activity has been met with limited success. This review focuses on the FACS screening of protein libraries for enzymatic activity.

引用

页码：321 / 328

页数：8

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