Is protein overlap in two-dimensional gels a serious practical problem?

被引：27

作者：

Hunsucker, SW

Duncan, MW

机构：

[1] Univ Colorado Denver, Dept Pediat, Sect Pulm Med, Aurora, CO 80045 USA

[2] Hlth Sci Ctr, Aurora, CO 80045 USA

[3] Univ Colorado Denver, Dept Med, Div Endocrinol Diabet & Metab, Aurora, CO 80045 USA

来源：

PROTEOMICS | 2006年 / 6卷 / 05期

关键词：

protein expression analysis; protein quantification; proteome dynamic range;

D O I：

10.1002/pmic.200500521

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

in a recently published article, Campostrini et al. [Proteomics 2005, 5, 2385-2395] raised questions regarding the utility of 2-D gels in proteomics research. We believe that the authors have overlooked several key issues including the dynamic range of protein expression and the sensitivity of the analytical methods used to explore a proteome. We argue that 2-D gels have and will continue to provide meaningful quantitative data when applied to proteomic analysis and that the practical significance of spot overlap has been overstated.

引用

收藏

页码：1374 / 1375

页数：2

相关论文

共 14 条

[1] Proteomic expression analysis of colorectal cancer by two-dimensional differential gel electrophoresis [J].

Alfonso, P ;

Núñez, A ;

Madoz-Gurpide, J ;

Lombardia, L ;

Sánchez, L ;

Casal, JI .

PROTEOMICS, 2005, 5 (10) :2602-2611

[2] The human plasma proteome - History, character, and diagnostic prospects [J].

Anderson, NL ;

Anderson, NG .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (11) :845-867

[3] Spot overlapping in two-dimensional maps: A serious problem ignored for much too long [J].

Campostrini, N ;

Areces, LB ;

Rappsilber, J ;

Pietrogrande, MC ;

Dondi, F ;

Pastorino, F ;

Ponzoni, M ;

Righetti, PG .

PROTEOMICS, 2005, 5 (09) :2385-2395

[4] Proteomic analysis of human serum by two-dimensional differential gel electrophoresis after depletion of high-abundant proteins [J].

Chromy, BA ;

Gonzales, AD ;

Perkins, J ;

Choi, MW ;

Corzett, MH ;

Chang, BC ;

Corzett, CH ;

McCutchen-Maloney, SL .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (06) :1120-1127

[5] Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry [J].

Friedman, DB ;

Hill, S ;

Keller, JW ;

Merchant, NB ;

Levy, SE ;

Coffey, RJ ;

Caprioli, RM .

PROTEOMICS, 2004, 4 (03) :793-811

[6] Differential diagnosis and non-surgical treatment of pelvic actinomycosis [J].

Nawroth, F ;

Foth, D ;

Schmidt, T ;

Römer, T .

ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 2000, 79 (11) :1024-1025

[7] Chromatofocusing fractionation and two-dimensional difference gel electrophoresis for low abundance serum proteins [J].

Qin, SZ ;

Ferdinand, AS ;

Richie, JP ;

O'Leary, MP ;

Mok, SC ;

Liu, BCS .

PROTEOMICS, 2005, 5 (12) :3183-3192

[8] High-throughput proteomic analysis of human infiltrating ductal carcinoma of the breast [J].

Somiari, RI ;

Sullivan, A ;

Russell, S ;

Somiari, S ;

Hu, H ;

Jordan, R ;

George, A ;

Katenhusen, R ;

Buchowiecka, A ;

Arciero, C ;

Brzeski, H ;

Hooke, J ;

Shriver, C .

PROTEOMICS, 2003, 3 (10) :1863-1873

[9] Protein profiling of human postmortem brain using 2-dimensional fluorescence difference gel electrophoresis (2-D DIGE) [J].

Swatton, JE ;

Prabakaran, S ;

Karp, NA ;

Lilley, KS ;

Bahn, S .

MOLECULAR PSYCHIATRY, 2004, 9 (02) :128-143

[10] Integrated genomic and proteomic analyses of gene expression in mammalian cells [J].

Tian, Q ;

Stepaniants, SB ;

Mao, M ;

Weng, L ;

Feetham, MC ;

Doyle, MJ ;

Yi, EC ;

Dai, HY ;

Thorsson, V ;

Eng, J ;

Goodlett, D ;

Berger, JP ;

Gunter, B ;

Linseley, PS ;

Stoughton, RB ;

Aebersold, R ;

Collins, SJ ;

Hanlon, WA ;

Hood, LE .

MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (10) :960-969