Using RT-PCR and bDNA assays to measure non-clade BHIV-1 subtype RNA

被引：20

作者：

Pasquier, C ^{[1
]}

Sandres, K ^{[1
]}

Salama, G ^{[1
]}

Puel, J ^{[1
]}

Izopet, J ^{[1
]}

机构：

[1] CHU Purpan, Virol Lab, F-31059 Toulouse, France

来源：

JOURNAL OF VIROLOGICAL METHODS | 1999年 / 81卷 / 1-2期

关键词：

HIV-1 RNA quantification; HIV-1; subtype; viral load;

D O I：

10.1016/S0166-0934(99)00057-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The performance of the new version of RT-PCR assay (Amplicor(TM) HIV-1 Monitor(TM) v1.5) was assessed. The quantification of non-B subtype HIV-1 plasma RNA (30A, 1C, 1D, 3E, 2F, 3G) obtained using Monitor(TM) v1.5 was compared to the former version of this assay (Monitor(TM) v1.0) and to the Quantiplex(TM) v2.0 bDNA assay. The new primers used in Monitor(TM) v1.5 were similar to the former version in both specificity and sensitivity. The new primers corrected the detection and quantification defect observed previously for HIV-1 non-B subtypes and gave slightly higher RNA concentrations than those measured using the bDNA assay (+ 0.39 log copies/ml). (C) Published by 1999 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 129

页数：7

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