Chemical approaches to synthetic biology

被引：47

作者：

Chiarabelli, Cristiano ^{[1
]}

Stano, Pasquale ^{[1
]}

Luisi, Pier Luigi ^{[1
]}

机构：

[1] Univ Roma TRE, Dept Biol, I-00146 Rome, Italy

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2009年 / 20卷 / 04期

关键词：

DE-NOVO PROTEINS; EXPRESSION; SELECTION; RNA; REPLICATION; LIPOSOMES; EVOLUTION; MICELLES; VESICLES; DESIGN;

D O I：

10.1016/j.copbio.2009.08.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The term chemical synthetic biology defines that part of the field that, instead of assuming an engineering approach based on genome manipulation, is oriented towards the synthesis of chemical structures alternative to those present in nature. Several different literature projects will be illustrated, including the two of our group. One is concerned with the 'Never Born Proteins' (NBPs), namely polypeptide sequences that are not present in nature, the other is concerned with the notion of,minimal cells', semi-synthetic compartments (usually liposomes) containing the minimal and sufficient number of components to perform the basic function of a biological cell.

引用

页码：492 / 497

页数：6

共 56 条

[31]

Ladner R C, 2000, Pharmacogenomics, V1, P199, DOI 10.1517/14622416.1.2.199

[32] Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis [J].