Evidence for a large compartment of IgM-expressing memory B cells in humans

被引:263
作者
Klein, U
Kuppers, R
Rajewsky, K
机构
关键词
D O I
10.1182/blood.V89.4.1288
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The recent finding of somatically mutated mu heavy chain transcripts in human peripheral blood (PB) B lymphocytes suggests that T-dependent B-cell memory might not be restricted to class-switched cells. We provide here evidence that IgM-only PB B cells are likely to be the IgM-expressing counterpart of classical (IgM(-)IgD(-)) memory B cells in humans. As shown by molecular single cell analysis, most IgM-only cells carry mutated V region genes, like class-switched cells. Although both subsets represent populations of nonactivated, resting cells, they express higher levels of Ig mRNA than naive (IgM(+)IgD(+)) B cells. IgM-only and class-switched cells are CD38(-)CD77(-), and mostly CD23(-), thus neither resembling germinal center nor naive B cells. Because many IgM-expressing B cells located in secondary lymphoid tissues resemble IgM-only PB B cells in terms of cell phenotype, we propose that the human lymphoid system contains a large compartment of IgM-expressing memory cells. Moreover, these cells seem to represent the nonmalignant counterparts of IgM-expressing tumor cells in sporadic Burkitt's lymphoma, MALT lymphoma, monocytoid B-cell lymphoma, and diffuse large-cell lymphoma that were found to harbor somatically mutated V genes. (C) 1997 by The American Society of Hematology.
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页码:1288 / 1298
页数:11
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