Analysis of the genetic variability of genes encoding the RNA III-activating components Agr and TRAP in a population of Staphylococcus aureus strains isolated from cows with mastitis

被引:220
作者
Gilot, P [1 ]
Lina, G
Cochard, T
Poutrel, B
机构
[1] INRA, Pathol Infect & Immunol Lab, INRA, F-37380 Nouzilly, France
[2] Ctr Natl Reference Toxemies Staphylocciques, Fac Med Laennec, F-69372 Lyon 08, France
关键词
D O I
10.1128/JCM.40.11.4060-4067.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The expression of Staphylococcus aureus virulence proteins is under the control of RNA 111, a central pleiotropic regulator transcribed from the agr locus. RNA III is activated by at least two two-component systems, one encoded by the agr locus (AgrC-AgrA) and another encoded outside of this locus (TRAP-RAP). In this work, we developed new typing methods based on genes encoding these two systems, which we used to characterize a nonclonal population of S. aureus bovine mastitis isolates. Twelve agr restriction types were identified in this population, but the majority of strains (56.3%) were grouped in the R III-A1 type. No strain isolated from humans, whose agr sequence is available from GenBank, was found to belong to this major type. Restriction maps constructed for all of those agr variants allowed the linking of all types in an evolution scheme and their grouping in one of the four agr interference groups. This analysis indicates that groups 2, 3, and 4 probably evolved from the more frequently encountered type, which belongs to group 1. agr group I was also found to be the most prevalent (69.0% of the strains) and the most polymorphic interference group. By developing an agr group-specific multiplex PCR, we confirmed the above classification of strains in the agr interference groups. Four allelic variants of trap were also identified, indicating that this two-component system is also polymorphic. The majority of strains was grouped in the trap I type (71.8%,). Whereas no relationships between agr group and trap types were found, strains of similar agr restriction type were also of similar trap type (with the exception of strains belonging to the agr R IV-A5 and R VI-A8 types). Our analysis indicates that S. aureus isolated from cows has predominantly a clonal structure and that the highly prevalent agr R III-A1, trap I type (56.3% of the strains) probably possesses a genetic background which endows it with superior ability to infect the bovine mammary gland.
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收藏
页码:4060 / 4067
页数:8
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