Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cells

被引：198

作者：

Lasne, David

Blab, Gerhard A.

Berciaud, Stephane

Heine, Martin

Groc, Laurent

Choquet, Daniel

Cognet, Laurent

Lounis, Brahim ^{[1
]}

机构：

[1] CNRS, Ctr Phys Mol Opt & Hertzienne, UMR 5798, Talence, France

[2] Univ Bordeaux 1, F-33405 Talence, France

[3] CNRS, UMR 5091, Bordeaux, France

[4] Univ Bordeaux 2, Inst Francois Magendie, F-33076 Bordeaux, France

来源：

BIOPHYSICAL JOURNAL | 2006年 / 91卷 / 12期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1529/biophysj.106.089771

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Tracking individual nano-objects in live cells during arbitrary long times is a ubiquitous need in modern biology. We present here a method for tracking individual 5-nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against single fluorescent molecule tracking in live COS7 cells on subsecond timescales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. Single Nanoparticle Photothermal Tracking has the unique potential to record arbitrary long trajectory of membrane proteins using nonfluorescent nanometer-sized labels.

引用

页码：4598 / 4604

页数：7

共 33 条

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