Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking

被引：1180

作者：

Dahan, M

Lévi, S

Luccardini, C

Rostaing, P

Riveau, B

Triller, A

机构：

[1] Ecole Normale Super, CNRS, UMR 8552, Lab Kastler Brossel, F-75005 Paris, France

[2] Univ Paris 06, F-75005 Paris, France

[3] Ecole Normale Super, INSERM, U497, F-75005 Paris, France

来源：

SCIENCE | 2003年 / 302卷 / 5644期

关键词：

D O I：

10.1126/science.1088525

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.

引用

页码：442 / 445

页数：4

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