Luminescent quantum dots for multiplexed biological detection and imaging

被引:2416
作者
Chan, WCW [1 ]
Maxwell, DJ
Gao, XH
Bailey, RE
Han, MY
Nie, SM
机构
[1] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[2] Georgia Inst Technol, Wallace Coulter Dept Biomed Engn, Atlanta, GA 30322 USA
[3] Emory Univ, Atlanta, GA 30322 USA
[4] Inst Mat Res & Engn, Singapore 117602, Singapore
[5] Natl Univ Singapore, Dept Mat Sci, Singapore 119260, Singapore
[6] Emory Univ, Sch Med, Dept Chem, Atlanta, GA 30322 USA
[7] Emory Univ, Sch Med, Dept Hematol & Oncol, Atlanta, GA 30322 USA
[8] Emory Univ, Sch Med, Winship Canc Inst, Atlanta, GA 30322 USA
关键词
D O I
10.1016/S0958-1669(02)00282-3
中图分类号
Q5 [生物化学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Recent advances in nanomaterials have produced a new class of fluorescent labels by conjugating semiconductor quantum dots with biorecognition molecules. These nanometer-sized conjugates are water-soluble and biocompatible, and provide important advantages over organic dyes and lanthanide probes. In particular, the emission wavelength of quantum-dot nanocrystals can be continuously tuned by changing the particle size, and a single light source can be used for simultaneous excitation of all different-sized dots. High-quality dots are also highly stable against photobleaching and have narrow, symmetric emission spectra. These novel optical properties render quantum dots ideal fluorophores for ultrasensitive, multicolor, and multiplexing applications in molecular biotechnology and bioengineering.
引用
收藏
页码:40 / 46
页数:7
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