On-line liquid-chromatography-nuclear magnetic resonance spectroscopy-mass spectrometry coupling for the separation and characterization of secoisolariciresinol diglucoside isomers in flaxseed

被引：34

作者：

Fritsche, J ^{[1
]}

Angoelal, R ^{[1
]}

Dachtler, M ^{[1
]}

机构：

[1] Unilever Res Labs, NL-3133 AT Vlaardingen, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 972卷 / 02期

关键词：

flaxseed; functional food; liquid chromatography-NMR spectroscopy mass spectrometry; nuclear magnetic resonance spectroscopy; secoisolariciresinol diglucoside;

D O I：

10.1016/S0021-9673(02)01110-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two secoisolariciresinol diglucoside (SDG) diastereomers were extracted from flaxseed and liberated through alkaline hydrolysis. Anion-exchange and reversed-phase chromatography were successfully employed to purify the hydrolyzed flaxseed extract. On-line LC-NMR-MS analyses revealed the structure of the isolated and purified SDG diastereomers, [2R,2'R]-2,3-bis[(4-hydroxy-3-methoxyphenyl)-methyl]-1,4-butanediyl-bis-beta-glucopyranoside the predominant flaxseed lignan and [2R,2'S]-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]-1,4-butanediyl-bis-beta-glucopyranoside, a previously incompletely characterized minor flaxseed lignan. Circular dichroism (CD) analyses confirmed the presence of two distinguished optically active compounds present in the flaxseed extract. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：195 / 203

页数：9

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