Application of staphylococcal enterotoxin B on normal and atopic skin induces up-regulation of T cells by a superantigen-mediated mechanism

被引:115
作者
Skov, L
Olsen, JV
Giorno, R
Schlievert, PM
Baadsgaard, O
Leung, DYM
机构
[1] Univ Copenhagen, Gentofte Hosp, Dept Dermatol, Copenhagen, Denmark
[2] Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80202 USA
[3] Univ Colorado, Hlth Sci Ctr, Dept Med, Denver, CO 80202 USA
[4] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA
关键词
superantigen; staphylococcal enterotoxin B; T-cell receptor V beta; atopic dermatitis;
D O I
10.1067/mai.2000.105524
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: The skin of patients with inflammatory skin diseases such as atopic dermatitis is frequently colonized with Staphylococcus aureus. Colonization with S aureus has been reported to exacerbate atopic dermatitis. Recent studies have demonstrated that S aureus isolated from the skin of patients with atopic dermatitis releases bacterial toxins that act as superantigens. We have previously applied the staphylococcal superantigen staphylococcal enterotoxin B (SEB) on intact human skin and found that the application led to induction of dermatitis. Objective: The purpose of the study was to determine whether superantigen-induced dermatitis is primarily due to a T cell-superantigen-mediated reaction or represents nonspecific cytokine-driven inflammation. Methods: We applied SEE, vehicle, and sodium lauryl sulfate on normal skin in healthy (n = 6) and atopic subjects (n = 6) and biopsy specimens were taken from all treated areas. The biopsy specimens from all subjects and peripheral blood from the atopic subjects were analyzed for the T-cell receptor (TCR) V beta repertoire with mAbs against TCR V beta 2, 3, 8.1, 12, 14, and 17. Results: From all subjects, both healthy and patients with atopic dermatitis, skin biopsy specimens from SEE-treated areas demonstrated selective accumulation of T cells expressing SEE-reactive TCR V beta 12 and 17 (P < .05). This selective up-regulation was not found in the sodium lauryl sulfate-treated areas. Conclusion: Our data strongly support that superantigen-induced T-cell activation is involved in the dermatitis seen after experimental application of SEE on intact skin.
引用
收藏
页码:820 / 826
页数:7
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