superantigen;
staphylococcal enterotoxin B;
T-cell receptor V beta;
atopic dermatitis;
D O I:
10.1067/mai.2000.105524
中图分类号:
R392 [医学免疫学];
学科分类号:
100102 ;
摘要:
Background: The skin of patients with inflammatory skin diseases such as atopic dermatitis is frequently colonized with Staphylococcus aureus. Colonization with S aureus has been reported to exacerbate atopic dermatitis. Recent studies have demonstrated that S aureus isolated from the skin of patients with atopic dermatitis releases bacterial toxins that act as superantigens. We have previously applied the staphylococcal superantigen staphylococcal enterotoxin B (SEB) on intact human skin and found that the application led to induction of dermatitis. Objective: The purpose of the study was to determine whether superantigen-induced dermatitis is primarily due to a T cell-superantigen-mediated reaction or represents nonspecific cytokine-driven inflammation. Methods: We applied SEE, vehicle, and sodium lauryl sulfate on normal skin in healthy (n = 6) and atopic subjects (n = 6) and biopsy specimens were taken from all treated areas. The biopsy specimens from all subjects and peripheral blood from the atopic subjects were analyzed for the T-cell receptor (TCR) V beta repertoire with mAbs against TCR V beta 2, 3, 8.1, 12, 14, and 17. Results: From all subjects, both healthy and patients with atopic dermatitis, skin biopsy specimens from SEE-treated areas demonstrated selective accumulation of T cells expressing SEE-reactive TCR V beta 12 and 17 (P < .05). This selective up-regulation was not found in the sodium lauryl sulfate-treated areas. Conclusion: Our data strongly support that superantigen-induced T-cell activation is involved in the dermatitis seen after experimental application of SEE on intact skin.